Simple and rapid detection of bacteria using a nuclease-responsive DNA probe

Cited 28 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorKyung Jin Lee-
dc.contributor.authorWang Sik Lee-
dc.contributor.authorAhreum Hwang-
dc.contributor.authorJeong Moon-
dc.contributor.authorTaejoon Kang-
dc.contributor.authorKyoungsook Park-
dc.contributor.authorJin Young Jeong-
dc.date.accessioned2020-08-25T09:39:04Z-
dc.date.available2020-08-25T09:39:04Z-
dc.date.issued2018-
dc.identifier.issn0003-2654-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/19950-
dc.description.abstractWe demonstrate simple and rapid bacterial detection using a nuclease-responsive DNA probe. The probe consisting of a fluorescent dye and a quencher at the 5′ and 3′ termini, respectively, was designed to be cleaved by nucleases such as endonucleases, exonucleases, and DNases, which are released from bacteria using an optimized lysis buffer. The fluorescence signal of the cleaved DNA probe correlates with the number of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, and the detection limit was 103 CFU for E. coli and 104 CFU for S. aureus. Moreover, this method is specific for live bacteria and takes just one minute to get the signal including sample collection. These features make the present bacterial detection method a powerful on-site bacterial contamination assay which is simple, rapid, and quantitative. ⓒ 2018 The Royal Society of Chemistry.-
dc.publisherRoyal Soc Chem-
dc.titleSimple and rapid detection of bacteria using a nuclease-responsive DNA probe-
dc.title.alternativeSimple and rapid detection of bacteria using a nuclease-responsive DNA probe-
dc.typeArticle-
dc.citation.titleAnalyst-
dc.citation.number0-
dc.citation.endPage338-
dc.citation.startPage332-
dc.citation.volume143-
dc.contributor.affiliatedAuthorKyung Jin Lee-
dc.contributor.affiliatedAuthorWang Sik Lee-
dc.contributor.affiliatedAuthorAhreum Hwang-
dc.contributor.affiliatedAuthorJeong Moon-
dc.contributor.affiliatedAuthorTaejoon Kang-
dc.contributor.affiliatedAuthorKyoungsook Park-
dc.contributor.affiliatedAuthorJin Young Jeong-
dc.contributor.alternativeName이경진-
dc.contributor.alternativeName이왕식-
dc.contributor.alternativeName황아름-
dc.contributor.alternativeName문정-
dc.contributor.alternativeName강태준-
dc.contributor.alternativeName박경숙-
dc.contributor.alternativeName정진영-
dc.identifier.bibliographicCitationAnalyst, vol. 143, pp. 332-338-
dc.identifier.doi10.1039/c7an01384a-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.