재설계 유전자 회로를 이용한 다양한 효소 활성의 탐색 및 정량방법

Cited 0 time in scopus
Metadata Downloads
Title
재설계 유전자 회로를 이용한 다양한 효소 활성의 탐색 및 정량방법
Alternative Title
Novel Method for Detecting and Quantitating Target Enzyme Activity Using Redesigned Genetic Circuitry
Inventor(s)
김희식이승구; 최수림; 나유진송재준최종현
Assignee(s)
한국생명공학연구원
Application No.
13/376,783 (2011.12.07)
Registration No.
9,783,859 (2017.10.10)
Country
미국
Abstract
A method of detecting and quantifying various enzymatic activities using a constructed artificial genetic circuit GESS (genetic enzyme screening system) for sensing phenolic compounds and a method of screening a trace of activities of target enzymes from a metagenome using the artificial genetic circuit, thereby securing target enzyme genes. When the method for screening and quantifying target enzymatic activity is used, useful genes can be screened from various genetic communities, including environmental or metagenomic libraries, at a single cell level in high throughput (million/day). Further, the sensitivity of the genetic circuit to phenol derivatives and the expression thereof can be controlled, and thus the genetic circuit can rapidly sense and quantify various enzymatic activities. Thus, the method can be advantageously used in the protein engineering technology for enzyme modification. Particularly, it can quantitatively investigate enzymatic activity, and thus can be applied to molecular evolution technology.
Type
Patent
Appears in Collections:
Division of Biomaterials Research > Cell Factory Research Center > 2. Patents
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 2. Patents
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 2. Patents
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.