생체 내 단백질 간 상호작용의 분석 방법

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Title
생체 내 단백질 간 상호작용의 분석 방법
Alternative Title
Method for detecting protein-protein interactions in cells
Inventor(s)
이승구; 이상준; 감종식; 최수림; 송재준
Assignee(s)
한국생명공학연구원
Application No.
13/524,868 (2012.06.15)
Registration No.
8,877,446 (2014.11.04)
Country
미국
Abstract
The present invention relates to a method for detecting protein-protein interactions in living cells, and more particularly, to a method for providing cells comprising a first construct and a second construct, wherein the first construct comprises a polynucleotide encoding a first fusion protein which comprises a bait protein, a first fluorescent protein and a CBD (cellulose-binding domain) protein, and wherein the second construct comprises a polynucleotide encoding a second fusion protein which comprises a prey protein and a second fluorescent protein so as to allow formation of inclusion bodies, and detecting interactions between the bait protein and the prey protein that are displayed by inclusion bodies, a method for isolating the prey protein bound to the bait protein using the cells comprising the constructs, the cells, and a kit for detecting protein-protein interactions, comprising the constructs.
Type
Patent
Appears in Collections:
Division of Biomaterials Research > 2. Patents
Division of Bio Technology Innovation > SME Support Center > 2. Patents
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