A designed whole-cell biosensor for live diagnosis of gut inflammation through nitrate sensing = 질산염 감지를 통한 장내 염증을 진단할 수 있는 디자인 미생물 개발

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dc.contributor.authorSeung Gyun Woo-
dc.contributor.authorSung-Je Moon-
dc.contributor.authorSeong Keun Kim-
dc.contributor.authorTae Hyun Kim-
dc.contributor.authorHyun Seung Lim-
dc.contributor.authorGun Hwi Yeon-
dc.contributor.authorBong Hyun Sung-
dc.contributor.authorChul Ho Lee-
dc.contributor.authorSeung Goo Lee-
dc.contributor.authorJung Hwan Hwang-
dc.contributor.authorDae-Hee Lee-
dc.date.accessioned2020-09-11T06:12:16Z-
dc.date.available2020-09-11T06:12:16Z-
dc.date.issued2020-
dc.identifier.issn09565663-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/21538-
dc.description.abstractMicrobes reprogrammed using advanced genetic circuits are envisaged as emerging living diagnostics for a wide range of diseases and play key roles in regulating gut microbiota to treat disease-associated symptoms in a non-invasive manner. Here, we developed a designer probiotic Escherichia coli that senses and responds to nitrate, a biomarker of gut inflammation. To this end, we first employed the NarX-NarL two-component regulatory system in E. coli to construct a nitrate-responsive genetic circuit. Next, we optimized the nitrate biosensor for the best performance using measures of sensitivity and specificity. We then introduced this genetic circuit into a probiotic E. coli Nissle 1917. We demonstrated that the designed biosensor can sense elevated nitrate levels during gut inflammation in mice with native gut microbiota. Moreover, using Boolean AND gate, we generated a genetically encoded biosensor for simultaneous sensing of the thiosulfate and nitrate biomarkers, thus increasing the tool's specificity for diagnosing gut inflammation. The nitrate-responsive genetic circuit will enable new approaches for non-invasive diagnostics of inflammation-associated diseases.-
dc.publisherElsevier-
dc.titleA designed whole-cell biosensor for live diagnosis of gut inflammation through nitrate sensing = 질산염 감지를 통한 장내 염증을 진단할 수 있는 디자인 미생물 개발-
dc.title.alternativeA designed whole-cell biosensor for live diagnosis of gut inflammation through nitrate sensing-
dc.typeArticle-
dc.citation.titleBiosensors & Bioelectronics-
dc.citation.number0-
dc.citation.endPage112523-
dc.citation.startPage112523-
dc.citation.volume168-
dc.contributor.affiliatedAuthorSeung Gyun Woo-
dc.contributor.affiliatedAuthorSung-Je Moon-
dc.contributor.affiliatedAuthorSeong Keun Kim-
dc.contributor.affiliatedAuthorTae Hyun Kim-
dc.contributor.affiliatedAuthorHyun Seung Lim-
dc.contributor.affiliatedAuthorGun Hwi Yeon-
dc.contributor.affiliatedAuthorBong Hyun Sung-
dc.contributor.affiliatedAuthorChul Ho Lee-
dc.contributor.affiliatedAuthorSeung Goo Lee-
dc.contributor.affiliatedAuthorJung Hwan Hwang-
dc.contributor.affiliatedAuthorDae-Hee Lee-
dc.contributor.alternativeName우승균-
dc.contributor.alternativeName문성제-
dc.contributor.alternativeName김성근-
dc.contributor.alternativeName김태현-
dc.contributor.alternativeName임현승-
dc.contributor.alternativeName연건휘-
dc.contributor.alternativeName성봉현-
dc.contributor.alternativeName이철호-
dc.contributor.alternativeName이승구-
dc.contributor.alternativeName황정환-
dc.contributor.alternativeName이대희-
dc.identifier.bibliographicCitationBiosensors & Bioelectronics, vol. 168, pp. 112523-112523-
dc.identifier.doi10.1016/j.bios.2020.112523-
dc.subject.keywordWhole cell biosensor-
dc.subject.keywordGut inflammation-
dc.subject.keywordDesigner probiotic-
dc.subject.keywordNitrate sensing-
dc.subject.keywordThiosulfate sensing-
dc.subject.keywordBoolean AND gate-
dc.subject.localWhole cell biosensor-
dc.subject.localGut inflammation-
dc.subject.localDesigner probiotic-
dc.subject.localNitrate sensing-
dc.subject.localThiosulfate sensing-
dc.subject.localBoolean AND gate-
dc.description.journalClassY-
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Laboratory Animal Resource Center > 1. Journal Articles
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