Multi-level stressor analysis from the DNA/biochemical level to community levels in an urban stream and integrative health response (IHR) assessments

Cited 4 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorJ H Lee-
dc.contributor.authorJ H Kim-
dc.contributor.authorHee-Mock Oh-
dc.contributor.authorK G An-
dc.date.accessioned2020-09-23T13:50:29Z-
dc.date.available2020-09-23T13:50:29Z-
dc.date.issued2013-
dc.identifier.issn1093-4529-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/22571-
dc.description.abstractThe objectives of this study were to identify multi-level stressors at the DNA/biochemical level to the community level in fish in an urban stream and to develop an integrative health response (IHR) model for ecological health diagnosis. A pristine control site (Sc) and an impacted site (S i) were selected from among seven pre-screened sites studied over seven years. Various chemical analyses indicated that nutrient enrichment (Nitrogen, Phosphorus) and organic pollution were significantly greater (t > 8.783, p < 0.01) at the Si site compared to the Sc site. Single-cell gel electrophoresis (comet assays) of DNA-level impairment indicated significantly (t = 5.678, p < 0.01) greater tail intensity, expressed as % tail-DNA, at the Si site and genotoxic responses were detected in the downstream reach. Ethoxyresorufin-O-deethylase (EROD) assays, as a physiological bioindicator, were 2.8-fold higher (p < 0.05, NK-test after ANOVA) at the Si site. Tissue analysis using a necropsy-based health assessment index (NHAI) showed distinct internal organ disorders in three tissues, i.e., liver, kidney, and gill, at the Si site. Population-level analysis using the sentinel species Zacco platypus showed that the regression coefficient (b) was 3.012 for the Si site and 2.915 for the Sc site, indicating population skewness in the downstream reach. Community-level health was impaired at the Si site based on an index of biological integrity (IBI), and physical habitat modifications were identified by a qualitative habitat evaluation index (QHEI). Overall, the model values for the integrative health response (IHR), developed using the star plot approach, were 3.22 (80.5%) at the Sc site and 0.74 (18.5%) at the Si site, indicating that, overall, ecological health impairments were evident in the urban reach. Our study was based on multi-level approaches using biological organization and the results suggest that there is a pivotal point of linkage between mechanistic understanding and real ecological consequences of environmental stressors. ⓒ Copyright Taylor and Francis Group, LLC.-
dc.publisherT&F (Taylor & Francis)-
dc.titleMulti-level stressor analysis from the DNA/biochemical level to community levels in an urban stream and integrative health response (IHR) assessments-
dc.title.alternativeMulti-level stressor analysis from the DNA/biochemical level to community levels in an urban stream and integrative health response (IHR) assessments-
dc.typeArticle-
dc.citation.titleJournal of Environmental Science and Health Part A-Toxic/Hazardous Substances & Environmental Engineering-
dc.citation.number2-
dc.citation.endPage222-
dc.citation.startPage211-
dc.citation.volume48-
dc.contributor.affiliatedAuthorHee-Mock Oh-
dc.contributor.alternativeName이재훈-
dc.contributor.alternativeName김준하-
dc.contributor.alternativeName오희목-
dc.contributor.alternativeName안광국-
dc.identifier.bibliographicCitationJournal of Environmental Science and Health Part A-Toxic/Hazardous Substances & Environmental Engineering, vol. 48, no. 2, pp. 211-222-
dc.identifier.doi10.1080/10934529.2012.717818-
dc.subject.keywordbiological integrity-
dc.subject.keywordcasual assessment-
dc.subject.keywordgenotoxic response-
dc.subject.keywordIntegrative health-
dc.subject.keywordmultimetric index-
dc.subject.keywordstressor-
dc.subject.localBiological integrity-
dc.subject.localbiological integrity-
dc.subject.localcasual assessment-
dc.subject.localgenotoxic response-
dc.subject.localIntegrative health-
dc.subject.localmultimetric index-
dc.subject.localstressor-
dc.description.journalClassY-
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.