Direct fermentation of Jerusalem artichoke tuber powder for production of L-lactic acid and D-lactic acid by metabolically engineered Kluyveromyces marxianus

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Title
Direct fermentation of Jerusalem artichoke tuber powder for production of L-lactic acid and D-lactic acid by metabolically engineered Kluyveromyces marxianus
Author(s)
Jung Hoon Bae; Hyun-Jin Kim; Mi Jin KimBong Hyun SungJae Heung JeonHyun Soon Kim; Y S Jin; D H Kweon; Jung Hoon Sohn
Bibliographic Citation
Journal of Biotechnology, vol. 266, pp. 27-33
Publication Year
2018
Abstract
An efficient production system for optically pure L- and D-lactic acid (LA) from Jerusalem artichoke tuber powder (JAP) was developed by metabolic engineering of Kluyveromyces marxianus. To construct LA-producing strains, the ethanol fermentation pathway of K. marxianus was redirected to LA production by disruption of KmPDC1 and expression of L- and D-lactate dehydrogenase (LDH) genes derived from Lactobacillus plantarum under the control of the K. marxianus translation elongation factor 1α promoter. To further increase the LA titer, the L-LA and D-LA consumption pathway of host strains was blocked by deletion of the oxidative LDH genes KmCYB2 and KmDLD1. The recombinant strains produced 130 g/L L-LA and 122 g/L D-LA by direct fermentation from 230 g/L JAP containing 140 g/L inulin, without pretreatment or nutrient supplementation. The conversion efficiency and optical purity were ≫>95% and ≫>99%, respectively. This system using JAP and the inulin-assimilating yeast K. marxianus could lead to a cost-effective process for the production of LA.
Keyword
InulinJerusalem artichokeKluyveromyces marxianusLactate dehydrogenaseLactic acid
ISSN
0168-1656
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.jbiotec.2017.12.001
Type
Article
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
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