Enhanced mating-type switching and sexual hybridization in heterothallic yeast Yarrowia lipolytica

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Title
Enhanced mating-type switching and sexual hybridization in heterothallic yeast Yarrowia lipolytica
Author(s)
Changpyo Han; Heeun Kwon; Gyuyeon Park; Minjeong JangHye Jeong LeeSunghwa Seo; Mincheol Kwon; Wooyoung Jeon; Heeseok Lee; Hong-Weon LeeJungoh Ahn
Bibliographic Citation
FEMS Yeast Research, vol. 20, no. 2, pp. foaa011-foaa011
Publication Year
2020
Abstract
Yarrowia lipolytica is a non-conventional, heterothallic, oleaginous yeast with wide range of industrial applications. Increasing ploidy can improve advantageous traits for industrial applications including genetic stability, stress resistance, and productivity, but the construction of knockout mutant strains from polyploid cells requires significant effort due to the increased copy numbers of target genes. The goal of this study was to evaluate the effectiveness of a mating-type switching strategy by single-step transformation without a genetic manipulation vestige, and to optimize the conventional method for increasing ploidy (mating) in Y. lipolytica. In this study, mating-type genes in haploid Y. lipolytica cells were scarlessly converted into the opposite type genes by site-specific homologous recombination, and the resulting MATB-type cells were mated at low temperature (22°C) with addition of sodium citrate with each MATA-type haploid cell to yield a MATA/MATB-type diploid strain with genetic information from both parental strains. The results of this study can be used to increase ploidy and for whole genome engineering of a yeast strain with unparalleled versatility for industrial application.
Keyword
Yarrowia lipolyticadiploidhaploidmating-type switchingoptimal yeast mating conditionssexual hybridization
ISSN
1567-1356
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1093/femsyr/foaa011
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
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