Cited 0 time in
- Enhanced mating-type switching and sexual hybridization in heterothallic yeast Yarrowia lipolytica
- Changpyo Han; Heeun Kwon; Gyuyeon Park; Minjeong Jang; Hye Jeong Lee; Sunghwa Seo; Mincheol Kwon; Wooyoung Jeon; Heeseok Lee; Hong-Weon Lee; Jungoh Ahn
- Bibliographic Citation
- FEMS Yeast Research, vol. 20, no. 2, pp. foaa011-foaa011
- Publication Year
- Yarrowia lipolytica is a non-conventional, heterothallic, oleaginous yeast with wide range of industrial applications. Increasing ploidy can improve advantageous traits for industrial applications including genetic stability, stress resistance, and productivity, but the construction of knockout mutant strains from polyploid cells requires significant effort due to the increased copy numbers of target genes. The goal of this study was to evaluate the effectiveness of a mating-type switching strategy by single-step transformation without a genetic manipulation vestige, and to optimize the conventional method for increasing ploidy (mating) in Y. lipolytica. In this study, mating-type genes in haploid Y. lipolytica cells were scarlessly converted into the opposite type genes by site-specific homologous recombination, and the resulting MATB-type cells were mated at low temperature (22°C) with addition of sodium citrate with each MATA-type haploid cell to yield a MATA/MATB-type diploid strain with genetic information from both parental strains. The results of this study can be used to increase ploidy and for whole genome engineering of a yeast strain with unparalleled versatility for industrial application.
- Yarrowia lipolytica; diploid; haploid; mating-type switching; optimal yeast mating conditions; sexual hybridization
- Oxford Univ Press
- Appears in Collections:
- Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
- Files in This Item:
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.