The protective effect of Leucosporidium-derived ice-binding protein (LeIBP) on bovine oocytes and embryos during vitrification

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Title
The protective effect of Leucosporidium-derived ice-binding protein (LeIBP) on bovine oocytes and embryos during vitrification
Author(s)
W S Sun; Hoon Jang; Hyo-Jin Kwon; Ki Young Kim; Soo Bin Ahn; S Hwang; S G Lee; J H Lee; I S Hwang; Jeong Woong Lee
Bibliographic Citation
Theriogenology, vol. 151, pp. 137-143
Publication Year
2020
Abstract
Ice-binding proteins (IBPs) facilitate organism survival under extreme conditions by inhibiting thermal hysteresis and ice recrystallization. IBPs have been widely used as cryoprotectants to cryopreserve mammalian gametes and embryos. In the present study, we evaluated the protective effects of an Arctic yeast, Leucosporidium sp. AY30 derived ice-binding protein (LeIBP), on the vitrification of bovine metaphase II (MII) oocytes and embryos. When oocytes and embryos were frozen using the two-step vitrification method, the survival rate was significantly increased in the presence of LeIBP. The LeIBP supplementation decreased the levels of intracellular reactive oxygen species (ROS) and enhanced mitochondrial functions in the vitrified?warmed oocytes. Furthermore, LeIBP improved the developmental potential and suppressed apoptosis of the embryos derived from vitrified?warmed oocytes. Collectively, these data indicate that LeIBP can be used as a promising cryoprotectant to prevent cryoinjury during vitrification in bovine oocytes. ⓒ 2020 The Authors
Keyword
Bovine oocyteEmbryo developmentLeIBPVitrification
ISSN
0093-691X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.theriogenology.2020.04.016
Type
Article
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
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