DC Field | Value | Language |
---|---|---|
dc.contributor.author | Su Ui Lee | - |
dc.contributor.author | Eun Sol Oh | - |
dc.contributor.author | Hyung Won Ryu | - |
dc.contributor.author | Mun-Ock Kim | - |
dc.contributor.author | Myung Ji Kang | - |
dc.contributor.author | Yu Na Song | - |
dc.contributor.author | Ro Woon Lee | - |
dc.contributor.author | Doo-Young Kim | - |
dc.contributor.author | H Ro | - |
dc.contributor.author | Sunin Jung | - |
dc.contributor.author | S T Hong | - |
dc.contributor.author | Sei-Ryang Oh | - |
dc.date.accessioned | 2020-09-24T03:09:54Z | - |
dc.date.available | 2020-09-24T03:09:54Z | - |
dc.date.issued | 2020 | - |
dc.identifier.issn | 1043-4666 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/22642 | - |
dc.description.abstract | Longifolioside A is an iridoid glucoside compound isolated from Pseudolysimachion rotundum var. subintegrum, which has been used in traditional herbal medicines to treat respiratory inflammatory diseases. Logifolioside A is a potent antioxidant; however, its underlying pharmacological mechanisms of action in inflammatory diseases are unknown. Here, we investigated the inhibitory effects of longifolioside A in lipopolysaccharide (LPS)-stimulated toll-like receptor 4 (TLR4) signal transduction systems using human THP-1 macrophages and HEK293 cells stably expressing human TLR4 protein (293/HA-hTLR4). Longifolioside A significantly reduced the release of inflammatory cytokines such as interleukin (IL)-6, -8, and tumor necrosis factor (TNF)-α in LPS-stimulated THP-1 macrophages. Furthermore, longifolioside A inhibited the expression of inflammatory mediator genes such as inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 that produce nitric oxide (NO) and prostaglandin E2 (PGE2), respectively. Longifolioside A suppressed the phosphorylation of PKCδ, IRAK4, IKKα/β, IκBα, and mitogen-activated protein (MAP) kinases (ERK 1/2 and JNK, but not p38), thereby inactivating the nuclear localization of NF-κB and AP-1, and thus decreasing the expression of inflammatory response genes. Notably, longifolioside A disrupted the interaction between human TLR4 and the TIR domain-containing adaptor protein (TIRAP), an early step during TLR4 activation, thereby reducing IL-8 secretion in 293/HA-hTLR4 cells. This inhibitory effect was comparable to that of TAK-242 (a TLR4 inhibitor, or resatorvid). Our results indicate that longifolioside A prevents inflammatory response by suppressing TLR4 activation required for NF-κB and AP-1 activation. | - |
dc.publisher | Elsevier | - |
dc.title | Longifolioside A inhibits TLR4-mediated inflammatory responses by blocking PKCδ activation in LPS-stimulated THP-1 macrophages | - |
dc.title.alternative | Longifolioside A inhibits TLR4-mediated inflammatory responses by blocking PKCδ activation in LPS-stimulated THP-1 macrophages | - |
dc.type | Article | - |
dc.citation.title | Cytokine | - |
dc.citation.number | 0 | - |
dc.citation.endPage | 155116 | - |
dc.citation.startPage | 155116 | - |
dc.citation.volume | 131 | - |
dc.contributor.affiliatedAuthor | Su Ui Lee | - |
dc.contributor.affiliatedAuthor | Eun Sol Oh | - |
dc.contributor.affiliatedAuthor | Hyung Won Ryu | - |
dc.contributor.affiliatedAuthor | Mun-Ock Kim | - |
dc.contributor.affiliatedAuthor | Myung Ji Kang | - |
dc.contributor.affiliatedAuthor | Yu Na Song | - |
dc.contributor.affiliatedAuthor | Ro Woon Lee | - |
dc.contributor.affiliatedAuthor | Doo-Young Kim | - |
dc.contributor.affiliatedAuthor | Sunin Jung | - |
dc.contributor.affiliatedAuthor | Sei-Ryang Oh | - |
dc.contributor.alternativeName | 이수의 | - |
dc.contributor.alternativeName | 오은솔 | - |
dc.contributor.alternativeName | 류형원 | - |
dc.contributor.alternativeName | 김문옥 | - |
dc.contributor.alternativeName | 강명지 | - |
dc.contributor.alternativeName | 송유나 | - |
dc.contributor.alternativeName | 이로운 | - |
dc.contributor.alternativeName | 김두영 | - |
dc.contributor.alternativeName | 노현주 | - |
dc.contributor.alternativeName | 정선인 | - |
dc.contributor.alternativeName | 홍성태 | - |
dc.contributor.alternativeName | 오세량 | - |
dc.identifier.bibliographicCitation | Cytokine, vol. 131, pp. 155116-155116 | - |
dc.identifier.doi | 10.1016/j.cyto.2020.155116 | - |
dc.subject.keyword | Inflammation | - |
dc.subject.keyword | Lipopolysaccharides | - |
dc.subject.keyword | Longifolioside A | - |
dc.subject.keyword | Toll-like receptor 4 | - |
dc.subject.keyword | Toll/interleukin-1 receptor domain-containing adapter protein | - |
dc.subject.local | Inflammation | - |
dc.subject.local | inflammation | - |
dc.subject.local | nflammation | - |
dc.subject.local | Lipopolysaccharide | - |
dc.subject.local | Lipopolysaccharide (LPS) | - |
dc.subject.local | Lipopolysaccharides | - |
dc.subject.local | lipopolysaccharide | - |
dc.subject.local | lipopolysaccharide (LPS) | - |
dc.subject.local | Longifolioside A | - |
dc.subject.local | TLR4 | - |
dc.subject.local | Toll-like receptor 4 | - |
dc.subject.local | Toll-like receptor 4 (TLR4) | - |
dc.subject.local | Toll-like-receptor4 | - |
dc.subject.local | Toll/interleukin-1 receptor domain-containing adapter protein | - |
dc.description.journalClass | Y | - |
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