Engineered Escherichia coli strains as platforms for biological production of isoprene

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Title
Engineered Escherichia coli strains as platforms for biological production of isoprene
Author(s)
Hyeok Won LeeJung Ho ParkWon-Kyo KimJin Gyeom LeeJu Seok LeeJungoh AhnEun Gyo LeeHong-Weon Lee
Bibliographic Citation
FEBS Open Bio, vol. 10, no. 5, pp. 780-788
Publication Year
2020
Abstract
Volatile compounds can be produced by fermentation from genetically engineered microorganisms. Escherichia?coli strains are mainly used for isoprene production owing to their higher titers; however, this has thus far been confined to only strains BL21, BL21 (DE3), Rosetta, and BW25113. Here, we tested four groups of E.?coli strains for improved isoprene production, including K-12 (DH5α, BW25113, W3110, MG1655, XL1-Blue, and JM109), B [Rosetta (DE3), BL21, and BL21 (DE3)], Crooks C, and Waksman W strains. The isoprene productivity of BL21 and MG1655 was remarkably higher than that of the others in 5-L fermentation, and scale-up fermentation (300?L) of BL21 was successfully performed. This system shows potential for biobased production of fuel and volatile compounds in industrial applications.
Keyword
Escherichia coli strainsfermentationisoprene productionscale-up
ISSN
2211-5463
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/2211-5463.12829
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Bio-Evaluation Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
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