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- Engineered Escherichia coli strains as platforms for biological production of isoprene
- Hyeok Won Lee; Jung Ho Park; Won-Kyo Kim; Jin Gyeom Lee; Ju Seok Lee; Jungoh Ahn; Eun Gyo Lee; Hong-Weon Lee
- Bibliographic Citation
- FEBS Open Bio, vol. 10, no. 5, pp. 780-788
- Publication Year
- Volatile compounds can be produced by fermentation from genetically engineered microorganisms. Escherichia？coli strains are mainly used for isoprene production owing to their higher titers; however, this has thus far been confined to only strains BL21, BL21 (DE3), Rosetta, and BW25113. Here, we tested four groups of E.？coli strains for improved isoprene production, including K-12 (DH5α, BW25113, W3110, MG1655, XL1-Blue, and JM109), B [Rosetta (DE3), BL21, and BL21 (DE3)], Crooks C, and Waksman W strains. The isoprene productivity of BL21 and MG1655 was remarkably higher than that of the others in 5-L fermentation, and scale-up fermentation (300？L) of BL21 was successfully performed. This system shows potential for biobased production of fuel and volatile compounds in industrial applications.
- Escherichia coli strains; fermentation; isoprene production; scale-up
- Appears in Collections:
- Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Bio-Evaluation Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
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