Overexpression of swpa4 peroxidase enhances tolerance to hydrogen peroxide and high salinity-mediated oxidative stress in transgenic sweetpotato plants

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Title
Overexpression of swpa4 peroxidase enhances tolerance to hydrogen peroxide and high salinity-mediated oxidative stress in transgenic sweetpotato plants
Author(s)
Y H Kim; Ho Soo KimSung Chul Park; Chang Yoon Ji; J W Yang; H U Lee; Sang Soo Kwak
Bibliographic Citation
Plant Biotechnology Reports, vol. 14, pp. 301-307
Publication Year
2020
Abstract
Secretory class III peroxidases are involved in plant responses to a range of biological stress conditions, including oxidative stress. To investigate the stress-related functions of the swpa4 peroxidase gene in sweetpotato (Ipomoea batatas L.), transgenic plants overexpressing the swpa4 gene under the control of the CaMV 35S promoter were generated using Agrobacterium-mediated transformation. Peroxidase activity was 3- to 13-fold higher in transgenic lines than in control plants. Transgenic plants were tested for tolerance to stress. Following treatment with 400mM hydrogen peroxide (H2O2), leaf discs from transgenic plants showed approximately 13-26% less damage than control plants. Transgenic plants also showed enhanced tolerance to high salinity conditions. Following treatment with NaCl, photosynthetic capacity and total chlorophyll contents were less severely impacted in swpa4 transgenic plants than in control plants. Aerial plant parts and storage root yields were not significantly different between transgenic and control plants following cultivation in field conditions. These results indicate that transgenic sweetpotato lines were able to respond efficiently to oxidative and saline stress via overexpression of swpa4.
Keyword
High salinityOxidative stressPeroxidaseReactive oxygen speciesSweetpotato
ISSN
1863-5466
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11816-020-00602-z
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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