Butylparaben is toxic to porcine oocyte maturation and subsequent embryonic development following in vitro fertilization

Cited 4 time in scopus
Metadata Downloads
Title
Butylparaben is toxic to porcine oocyte maturation and subsequent embryonic development following in vitro fertilization
Author(s)
Pil Soo Jeong; Sanghoon Lee; Soo-Hyun Park; Min Ju Kim; Hyogu Kang; T Nanjidsuren; Hee Chang SonBong-Seok Song; D B Koo; Bo Woong SimSun-Uk Kim
Bibliographic Citation
International Journal of Molecular Sciences, vol. 21, pp. 3692-3692
Publication Year
2020
Abstract
Parabens are widely used in personal care products due to their antimicrobial effects. Although the toxicity of parabens has been reported, little information is available on the toxicity of butylparaben (BP) on oocyte maturation. Therefore, we investigated the effects of various concentrations of BP (0 μM, 100 μM, 200 μM, 300 μM, 400 μM, and 500 μM) on the in vitro maturation of porcine oocytes. BP supplementation at a concentration greater than 300 μM significantly reduced the proportion of complete cumulus cell expansion and metaphase II oocytes compared to the control. The 300 μM BP significantly decreased fertilization, cleavage, and blastocyst formation rates with lower total cell numbers and a higher rate of apoptosis in blastocysts compared to the control. The BP-treated oocytes showed significantly higher reactive oxygen species (ROS) levels, and lower glutathione (GSH) levels than the control. BP significantly increased the aberrant mitochondrial distribution and decreased mitochondrial function compared to the control. BP-treated oocytes exhibited significantly higher percentage of γ-H2AX, annexin V-positive oocytes and expression of LC3 than the control. In conclusion, we demonstrated that BP impaired oocyte maturation and subsequent embryonic development, by inducing ROS generation and reducing GSH levels. Furthermore, BP disrupted mitochondrial function and triggered DNA damage, early apoptosis, and autophagy in oocytes.
Keyword
ApoptosisButylparabenIn vitro maturationMitochondriaPorcine oocyteROS
ISSN
1422-0067
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/ijms21103692
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Files in This Item:

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.