Identification and characterization of a cocoon degradable enzyme from the isolated strain Bacillus subtilis Bs5C

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Title
Identification and characterization of a cocoon degradable enzyme from the isolated strain Bacillus subtilis Bs5C
Author(s)
Hyun Deok Oh; Min Seo Cho; Joong Su KimMin-Soo KimChul Ho KimJi Young Kang
Bibliographic Citation
Biotechnology and Bioprocess Engineering, vol. 25, no. 3, pp. 442-449
Publication Year
2020
Abstract
A fibroin-degrading bacterium was isolated in a medium containing fibroin as a sole nitrogen source, and then identified and characterized. The strain was designated Bacillus subtilis strain Bs5C, and the culture medium containing the extracellular proteolytic enzymes was found to hydrolyze cocoon fiber. A decomposition rate of 35% was achieved with the culture medium in optimized cocoon-degrading condition. In a second round of decomposition performed on the same fibers with additional culture medium, the decomposition rate reached 45.9%. To investigate the proteolytic enzymes degrading cocoon, the enzymes was purified from culture medium using ion exchange column and identified using LC-MS/MS analysis system. As a result, it was uncovered that the cocoon degradation was due to the neutral protease NprE of the strain Bs5C. Moreover, the cocoon fibers treated with semi-purified NprE enzyme solution were clearly split and degraded, as assessed by SEM, and solubilized peptides from fibroin and sericin were detected by LC-MS/MS. In conclusion, this study is the first report that peptides could be produced from cocoon by cultured medium of B. subtilis, and the newly isolated strain Bs5C and the NprE protease from the strain Bs5C could clearly be valuable for the production of silk peptides, which have various pharmacological and industrial applications. ⓒ 2020, The Korean Society for Biotechnology and Bioengineering and Springer.
Keyword
Bacillus subtilis strain Bs5Ccocoon decompositionsilk peptideneutral protease
ISSN
1226-8372
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s12257-019-0399-5
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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