Chaetocin improves pig cloning efficiency by enhancing epigenetic reprogramming and autophagic activity

Cited 9 time in scopus
Metadata Downloads
Title
Chaetocin improves pig cloning efficiency by enhancing epigenetic reprogramming and autophagic activity
Author(s)
Pil Soo Jeong; Bo Woong Sim; Soo-Hyun Park; Min Ju Kim; Hyo-Gu Kang; T Nanjidsuren; Sanghoon Lee; Bong-Seok Song; D B Koo; Sun-Uk Kim
Bibliographic Citation
International Journal of Molecular Sciences, vol. 21, no. 14, pp. 4836-4836
Publication Year
2020
Abstract
Efficient epigenetic reprogramming is crucial for the in vitro development of mammalian somatic cell nuclear transfer (SCNT) embryos. The aberrant levels of histone H3 lysine 9 trimethylation (H3K9me3) is an epigenetic barrier. In this study, we evaluated the effects of chaetocin, an H3K9me3-specific methyltransferase inhibitor, on the epigenetic reprogramming and developmental competence of porcine SCNT embryos. The SCNT embryos showed abnormal levels of H3K9me3 at the pronuclear, two-cell, and four-cell stages compared to in vitro fertilized embryos. Moreover, the expression levels of H3K9me3-specific methyltransferases (suv39h1 and suv39h2) and DNA methyltransferases (DNMT1, DNMT3a, and DNMT3b) were higher in SCNT embryos. Treatment with 0.5 nM chaetocin for 24 h after activation significantly increased the developmental competence of SCNT embryos in terms of the cleavage rate, blastocyst formation rate, hatching rate, cell number, expression of pluripotency-related genes, and cell survival rate. In particular, chaetocin enhanced epigenetic reprogramming by reducing the H3K9me3 and 5-methylcytosine levels and restoring the abnormal expression of H3K9me3-specific methyltransferases and DNA methyltransferases. Chaetocin induced autophagic activity, leading to a significant reduction in maternal mRNA levels in embryos at the pronuclear and two-cell stages. These findings revealed that chaetocin enhanced the developmental competence of porcine SCNT embryos by regulating epigenetic reprogramming and autophagic activity and so could be used to enhance the production of transgenic pigs for biomedical research.
Keyword
chaetocinepigenetic reprogrammingH3K9me3DNA methylationporcine SCNT embryoautophagy
ISSN
1422-0067
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/ijms21144836
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Files in This Item:

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.