Phylogenetic analysis of endangered wild animal class II Satsuma myomphala using the argrinine kinase gene = 멸종위기야생동물 Ⅱ급 Satsuma myomphala (거제외줄달팽이) 의 argrinine kinase 유전자를 이용한 계통학적 분석

Abstract

Arginine kinase (AK) belongs to the phosphagen kinase (PK) super family group of enzyme that has an essential role in maintaining energy homeostasis by catalyzing the re-phosphorylation of ADP. The AK gene is used for phylogenetic analysis in arthropods, mollusks, and Nematoda, and has been reported to branch in the Phylum unit. Satsuma myomphala has been reported as an indigenous species in Northeast Asia, including Korea and Japan. Particularly in Korea, it was registered as an endangered wildlife class 2 in 2017. Therefore, the preservation of species and securing genetic resources became more important. The genetic resources of S. myomphala registered on the NCBI GenBank have only six nucleotides and two proteins registered. The AK sequence was extracted from the RNAseq data which was obtained from Illumina Hiseq 2500 platfrom. After sequencing, de novo assembly and clustering, 103,774 unigenes were generated. Through annotation step by using the PANM database and BLAST program, we obtained AK sequence of 354 amino acid residues containing a 1,062 bp coding region. The AK sequence was verified using the BLAST program in the NCBI nr database, and phylogenetic analysis with the AK sequences of Gastropoda, Bivalvia, and Cephalopoda registered in NCBI was conducted. A phylogenetic dendrogram was generated using the MEGA program, and we confirmed the phylogenetic relationships. In addition, the 2D and 3D structures of the AK gene were predicted.