Inhibitory effects of orientin in mast cell-mediated allergic inflammation

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Inhibitory effects of orientin in mast cell-mediated allergic inflammation
H Dhakal; Soyoung Lee; J K Choi; T K Kwon; D Khang; S H Kim
Bibliographic Citation
Pharmacological Reports, vol. 72, no. 4, pp. 1002-1010
Publication Year
Background: Mast cells are immune effector cells mediating allergic inflammation by the secretion of inflammatory mediators such as histamine and pro-inflammatory cytokines. Orientin is a naturally occurring bioactive flavonoid that possesses diverse biological properties, including anti-inflammation, anti-oxidative, anti-tumor, and cardio protection. The objective of this study was to rule out the effectiveness of orientin in mast cell-mediated allergic inflammation. Methods: In this study, in vitro effects of orientin were evaluated in RBL-2H3, mouse bone marrow-derived mast cells, rat peritoneal mast cells, and in vivo effects were evaluated by inducing passive cutaneous anaphylaxis (PCA) in Imprinting Control Region (ICR) mice. Results: Findings show that orientin suppressed the immunoglobulin E (IgE)-mediated mast cell degranulation by reducing intracellular calcium level in a concentration-dependent manner. Orientin suppressed the secretion of pro-inflammatory cytokines in mast cells. This inhibitory effects of orientin was through inhibition of FcεRI-mediated signaling proteins. In addition, oral administration of orientin suppressed the IgE-mediated PCA reactions in a dose-dependent manner, which was evidenced by reduced Evan's blue pigmentation and ear swelling. Conclusions: Based on these findings, we suggest that orientin might have potential to alleviate allergic reaction and mast cell-mediated allergic disease.
Mast cellAllergic inflammationHistaminePassive cutaneous anaphylaxisOrientin
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Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
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