Genetic impairment of cellulose biosynthesis increases cell wall fragility and improves lipid extractability from oleaginous alga Nannochloropsis salina

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Title
Genetic impairment of cellulose biosynthesis increases cell wall fragility and improves lipid extractability from oleaginous alga Nannochloropsis salina
Author(s)
S W Jeong; Kwon Hwangbo; Jong Min Lim; S W Nam; B S Lee; B R Jeong; Y K Chang; Won Joong Jeong; Y I Park
Bibliographic Citation
Microorganisms, vol. 8, no. 8, pp. 1195-1195
Publication Year
2020
Abstract
In microalgae, photosynthesis provides energy and sugar phosphates for the biosynthesis of storage and structural carbohydrates, lipids, and nitrogenous proteins. The oleaginous alga Nannochloropsis salina does not preferentially partition photoassimilates among cellulose, chrysolaminarin, and lipids in response to nitrogenous nutrient deprivation. In the present study, we investigated whether genetic impairment of the cellulose synthase gene (CesA) expression would lead to protein accumulation without the accumulation of storage C polymers in N. salina. Three cesA mutants were generated by the CRISPR/Cas9 approach. Cell wall thickness and cellulose content were reduced in the cesA1 mutant, but not in cesA2 or cesA4 cells. CesA1 mutation resulted in a reduction of chrysolaminarin and neutral lipid contents, by 66.3% and 37.1%, respectively, but increased the soluble protein content by 1.8-fold. Further, N. salina cells with a thinned cell wall were susceptible to mechanical stress, resulting in a 1.7-fold enhancement of lipid extractability. Taken together, the previous and current studies strongly suggest the presence of a controlling mechanism that regulates photoassimilate partitioning toward C and N metabolic pathways as well as the cellulose metabolism as a potential target for cost-effective microalgal cell disruption and as a useful protein production platform.
Keyword
cell wallcellulose synthase CesAcesA mutantCRISPR/Cas9photosynthate partitioningNannochloropsis
ISSN
2076-2607
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/microorganisms8081195
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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