Induction of glucoraphasatin biosynthesis genes by MYB29 in radish ( Raphanus sativus L.) roots

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Title
Induction of glucoraphasatin biosynthesis genes by MYB29 in radish ( Raphanus sativus L.) roots
Author(s)
J N Kang; S Y Won; M S Seo; Jeongyeo Lee; S M Lee; S J Kwon; J S Kim
Bibliographic Citation
International Journal of Molecular Sciences, vol. 21, no. 16, pp. 5721-5721
Publication Year
2020
Abstract
Glucoraphasatin (GRH) is a specific aliphatic glucosinolate (GSL) that is only abundant in radish (Raphanus sativus L.). The gene expression regulating GRH biosynthesis in radish is still poorly understood. We employed a total of 59 radish accessions to analyze GSL profiles and showed that GRH was specific and predominant among the aliphatic GSLs in radish roots. We selected five accessions roots with high, moderate and low GSL biosynthesis, respectively, to conduct a comparative transcriptome analysis and the qRT-PCR of the biosynthesis genes for aliphatic GSLs. In this study, among all the accessions tested, roots with the accession RA157-74 had a high GRH content and showed a significant expression of the aliphatic GSL biosynthesis genes. We defined the genes involved in the GRH biosynthesis process and found that they were regulated by a transcription factor (RSG00789) at the MYB29 locus in radish roots. We found 13 aliphatic GSL biosynthesis genes regulated by the RSG00789 gene in the GRH biosynthesis pathway.
Keyword
glucoraphasatinglucosinolatesMYB transcription factorsradish transcriptomeGRS1
ISSN
1422-0067
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/ijms21165721
Type
Article
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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