Selective elimination of human pluripotent stem cells by Anti-Dsg2 antibody-doxorubicin conjugates

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Title
Selective elimination of human pluripotent stem cells by Anti-Dsg2 antibody-doxorubicin conjugates
Author(s)
Jongjin Park; Na Geum Lee; Mihee Oh; Jinhoi Song; Wooil Kim; Min-Gi Kwon; Seul Gi Kim; Baek Soo HanKwang-Hee BaeDong Gwang LeeSang Hyun LeeJong Gil Park; J H Kim; Jangwook LeeJeong Ki Min
Bibliographic Citation
Biomaterials, vol. 259, pp. 120265-120265
Publication Year
2020
Abstract
The self-renewal properties of human pluripotent stem cells (hPSCs) contribute to their efficacy in tissue regeneration applications yet increase the likelihood of teratoma formation, thereby limiting their clinical utility. To address this issue, we developed a tool to specifically target and neutralize undifferentiated hPSCs, thereby minimizing tumorigenicity risk without negatively affecting regenerated and somatic tissues. Specifically, we conjugated a monoclonal antibody (K6-1) previously generated in our laboratory against desmoglein 2 (Dsg2), which is highly differentially expressed in undifferentiated hPSCs versus somatic tissues, to the chemotherapeutic agent doxorubicin (DOX). The K6-1-DOX conjugates were selectively targeted and incorporated into Dsg2-positive hPSCs, leading to pH-dependent endosomal release and nuclear localization of DOX with subsequent cytotoxicity via an apoptotic caspase cascade. Conversely, Dsg2-negative fibroblasts showed minimal conjugate uptake or cytotoxicity, suggesting that K6-1-DOX treatment would yield few side effects owing to off-target effects. Selective removal of undifferentiated stem cells was also supported by in vivo studies using a mouse xenograft model, wherein hIgG-DOX- but not K6-1-DOX-pretreated-hPSC injection led to teratoma development. Together, these results validated the ability of the Dsg2-targeted antibody-anticancer drug conjugate to facilitate the safety of stem cell therapies.
Keyword
Antibody-drug conjugatesTargeted deliveryPluripotent stem cellDesmoglein-2Teratoma elimination
ISSN
0142-9612
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.biomaterials.2020.120265
Type
Article
Appears in Collections:
Division of Research on National Challenges > Biodefense Research Center > 1. Journal Articles
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
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