Vitrification for cryopreservation of 2D and 3D stem cells culture using high concentration of cryoprotective agents

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Title
Vitrification for cryopreservation of 2D and 3D stem cells culture using high concentration of cryoprotective agents
Author(s)
Y H Jeong; E Kim; S G Lee; B Ryu; J Kim; A Igor; J S Kim; Jung Cho Rok; J H Park; C Y Kim
Bibliographic Citation
BMC Biotechnology, vol. 20, pp. 45-45
Publication Year
2020
Abstract
Background: Vitrification is the most promising technology for successful cryopreservation of living organisms without ice crystal formation. However, high concentrations (up to ~ 6-8 M) of cryoprotective agents (CPAs) used in stem cell induce osmotic and metabolic injuries. Moreover, the application of conventional slow-freezing methods to cultures of 3-D organoids of stem cells in various studies, is limited by their size. Results: In this study, we evaluated the effect of high concentrations of CPAs including cytotoxicity and characterized human mesenchymal stem cell (MSC) at single cell level. The cell viability, cellular damage, and apoptotic mechanisms as well as the proliferation capacity and multipotency of cells subjected to vitrification were similar to those in the slow-freezing group. Furthermore, we identified the possibility of vitrification of size-controlled 3-D spheroids for cryopreservation of organoid with high survivability. Conclusions: Our results demonstrate successful vitrification of both single cell and spheroid using high concentration of CPAs in vitro without cytotoxicity.
Keyword
VitrificationCryoprotective agentIn vitroMSCSpheroid
ISSN
1472-6750
Publisher
Springer-BMC
DOI
http://dx.doi.org/10.1186/s12896-020-00636-9
Type
Article
Appears in Collections:
Division of Research on National Challenges > Stem Cell Convergenece Research Center > 1. Journal Articles
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