DNA data storage in Perl

Cited 1 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorUi Jin Lee-
dc.contributor.authorSeulki Hwang-
dc.contributor.authorK E Kim-
dc.contributor.authorMoonil Kim-
dc.date.accessioned2020-09-24T04:05:33Z-
dc.date.available2020-09-24T04:05:33Z-
dc.date.issued2020-
dc.identifier.issn12268372-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/22798-
dc.description.abstractHere we report a simple and flexible method for DNA data storage based on Perl script. For this approach, the text data of the preamble of the “Universal Declaration of Human Rights” consisting of 2,046 words was encoded into the corresponding 8,148 base pairs of DNA using Perl-based encoding with a hash table. The encoded DNA sequences were then artificially synthesized for storage. The information DNA consisted of a total of 22 chemically synthesized DNA fragments with 400 nucleotides each, which were inserted into a cloning vector to multiply the plasmid DNA. The nucleotide integrity of the data-carrying DNA sequences were ensured under the accelerated aging conditions. Also, an erroneous nucleotide in the information DNA sequences was successfully corrected using the overlap extension PCR method. The stored DNA was read by sequencing, and the resulting DNA sequence information was successfully decoded to convert the DNA records back to the original document. Our results indicate that textual data can be stored in DNA using a simple, easy, and flexible Perl by running a script from the command line.-
dc.publisherSpringer-
dc.titleDNA data storage in Perl-
dc.title.alternativeDNA data storage in Perl-
dc.typeArticle-
dc.citation.titleBiotechnology and Bioprocess Engineering-
dc.citation.number0-
dc.citation.endPage615-
dc.citation.startPage607-
dc.citation.volume25-
dc.contributor.affiliatedAuthorUi Jin Lee-
dc.contributor.affiliatedAuthorSeulki Hwang-
dc.contributor.affiliatedAuthorMoonil Kim-
dc.contributor.alternativeName이의진-
dc.contributor.alternativeName황슬기-
dc.contributor.alternativeName김균언-
dc.contributor.alternativeName김문일-
dc.identifier.bibliographicCitationBiotechnology and Bioprocess Engineering, vol. 25, pp. 607-615-
dc.identifier.doi10.1007/s12257-020-0022-9-
dc.subject.keywordDNA-
dc.subject.keywordDNA data storage-
dc.subject.keywordPerl-
dc.subject.keywordoverlap extension PCR-
dc.subject.localDNA-
dc.subject.localDNA data storage-
dc.subject.localPerl-
dc.subject.localoverlap extension PCR-
dc.description.journalClassY-
Appears in Collections:
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.