Antibody-secreting macrophages generated using CpG-free plasmid eliminate tumor cells through antibody-dependent cellular phagocytosis

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Title
Antibody-secreting macrophages generated using CpG-free plasmid eliminate tumor cells through antibody-dependent cellular phagocytosis
Author(s)
Eun Bi Cha; Keun Koo Shin; Jinho SeoDoo-Byoung Oh
Bibliographic Citation
BMB Reports, vol. 53, no. 8, pp. 442-447
Publication Year
2020
Abstract
The non-viral delivery of genes into macrophages, known as hard-to-transfect cells, is a challenge. In this study, the microporation of a CpG-free and small plasmid (pCGfd-GFP) showed high transfection efficiency, sustainable transgene expression, and good cell viability in the transfections of Raw 264.7 and primary bone marrow-derived macrophages. The non-viral method using the pCGfd vector encoding anti-EGFR single-chain Fv fused with Fc (scFv-Fc) generated the macrophages secreting anti-EGFR scFv-Fc. These macrophages effectively phagocytized tumor cells expressing EGFR through the antibody-dependent mechanism, as was proved by experiments using EGFR-knockout tumor cells. Finally, peri-tumoral injections of anti-EGFR scFv-Fc-secreting macrophages were shown to inhibit tumor growth in the xenograft mouse model.
Keyword
CpG-free plasmidMacrophagesNon-viral gene deliveryAntibody-secreting macrophageAntibody-dependent cellular phagocytosis
ISSN
1976-6696
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5483/BMBRep.2020.53.8.024
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
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