The Gata1low murine megakaryocyte-erythroid progenitor cells expand robustly and alter differentiation potential

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dc.contributor.authorEunju Shin-
dc.contributor.authorJong Gwan Jeong-
dc.contributor.authorHyunmin Chung-
dc.contributor.authorHaiyoung Jung-
dc.contributor.authorC Park-
dc.contributor.authorSuk Ran Yoon-
dc.contributor.authorTae-Don Kim-
dc.contributor.authorS J Lee-
dc.contributor.authorInpyo Choi-
dc.contributor.authorJi Yoon Noh-
dc.date.accessioned2020-10-27T03:16:46Z-
dc.date.available2020-10-27T03:16:46Z-
dc.date.issued2020-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/22978-
dc.description.abstractGATA1 is a master transcription factor of megakaryopoiesis and erythropoiesis, and loss-of-function mutation can induce accumulation of megakaryocyte-erythroid progenitors (MEPs) in mice and humans. Accordingly, the murine MEP cell line (termed G1ME2 cells) encoding doxycycline (dox)-inducible anti-Gata1 shRNA on Hprt locus has been developed. The cells were CD41+CD71+KIT+, expand under dox, stem cell factor, and thrombopoietin (TPO), and terminally differentiate into erythroid cells or megakaryocytes upon removal of dox. Surprisingly, in this study, these Gata1low murine MEPs displayed accelerated growth from around 90-100 days after cell culture, impeded megakaryocytic potential, and maintained erythropoiesis. We specified them as late G1ME2 cells and discovered that increased CD41-KIT+ population during long-term culture was the main reason for the delayed megakaryopoiesis. The CD41 expression level was partially de-repressed by PI3K/AKT inhibitors, suggesting that TPO-mediated cell survival signaling pathway might have impacted on CD41 in the late G1ME2 cells. Nevertheless, among the late cells, the CD41+KIT+ cells could still generate megakaryocytes on dox withdrawal. Taken together, G1ME2 cells could provide a good model to study molecular mechanism of hematopoiesis because of their ability to expand excessively without artificial immortalization.-
dc.publisherElsevier-
dc.titleThe Gata1low murine megakaryocyte-erythroid progenitor cells expand robustly and alter differentiation potential-
dc.title.alternativeThe Gata1low murine megakaryocyte-erythroid progenitor cells expand robustly and alter differentiation potential-
dc.typeArticle-
dc.citation.titleBiochemical and Biophysical Research Communications-
dc.citation.number0-
dc.citation.endPage53-
dc.citation.startPage46-
dc.citation.volume528-
dc.contributor.affiliatedAuthorEunju Shin-
dc.contributor.affiliatedAuthorJong Gwan Jeong-
dc.contributor.affiliatedAuthorHyunmin Chung-
dc.contributor.affiliatedAuthorHaiyoung Jung-
dc.contributor.affiliatedAuthorSuk Ran Yoon-
dc.contributor.affiliatedAuthorTae-Don Kim-
dc.contributor.affiliatedAuthorInpyo Choi-
dc.contributor.affiliatedAuthorJi Yoon Noh-
dc.contributor.alternativeName신은주-
dc.contributor.alternativeName정종관-
dc.contributor.alternativeName정현민-
dc.contributor.alternativeName정해용-
dc.contributor.alternativeName박찬이-
dc.contributor.alternativeName윤석란-
dc.contributor.alternativeName김태돈-
dc.contributor.alternativeName이승진-
dc.contributor.alternativeName최인표-
dc.contributor.alternativeName노지윤-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, vol. 528, pp. 46-53-
dc.identifier.doi10.1016/j.bbrc.2020.04.143-
dc.subject.keywordMegakaryocyte-erythroid progenitor-
dc.subject.keywordCD41-
dc.subject.keywordGATA1-
dc.subject.keywordCell proliferation-
dc.subject.keywordMegakaryopoiesis-
dc.subject.localMegakaryocyte-erythroid progenitor-
dc.subject.localmegakaryocyte-erythroid progenitor-
dc.subject.localCD41-
dc.subject.localGATA-1-
dc.subject.localGATA1-
dc.subject.localCell proliferation-
dc.subject.localcell proliferation-
dc.subject.localMegakaryopoiesis-
dc.description.journalClassY-
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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