Effects of Leucosporidium-derived ice-binding protein (LeIBP) on bull semen cryopreservation

Cited 5 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorHoon Jang-
dc.contributor.authorHyo-Jin Kwon-
dc.contributor.authorW S Sun-
dc.contributor.authorS Hwang-
dc.contributor.authorI S Hwang-
dc.contributor.authorS Kim-
dc.contributor.authorJ H Lee-
dc.contributor.authorS G Lee-
dc.contributor.authorJeong Woong Lee-
dc.date.accessioned2020-10-27T03:27:12Z-
dc.date.available2020-10-27T03:27:12Z-
dc.date.issued2020-
dc.identifier.issn20531095-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/23010-
dc.description.abstractWe examined the effect of ice-binding protein derived from Leucosporidium (LeIBP) on the cryopreservation of bull semen and compared it with that derived from previously reported Antifreeze Protein III (AFPIII). Six concentrations of LeIBP (10-1 ~ 104 μg/ml) and AFPIII (10-1 ~ 104 μg/ml) were added to the bull semen extender, respectively. Sperm kinematic parameters were measured to examine sperm toxicity and cryopreserved sperm quality. Measures of antioxidant activity such as superoxide dismutase (SOD), reduced glutathione/oxidative glutathione (GSH/GSSG), and total antioxidant capacity (TAC) were analysed to identify the effect of LeIBP on sperm quality. In addition, sperm viability was analysed using a flow cytometer and fluorescence microscope by SYBR14/PI staining. The results showed that the LeIBP groups (0.1, 1 and 10 μg/ml) were less toxic, and the quality of the sperm were dramatically improved in the extenders containing 0.1 μg/ml LeIBP among concentrations of LeIBP and AFPIII. The SOD activity of LeIBP was greater than that of AFPIII and control. In addition, sperm viability was enhanced in the LeIBP-treated group. In summary, LeIBP is a useful cryoprotective adjuvant for bull sperm cryopreservation, and the most efficient concentration of LeIBP is 0.1 μg/ml.-
dc.publisherWiley-
dc.titleEffects of Leucosporidium-derived ice-binding protein (LeIBP) on bull semen cryopreservation-
dc.title.alternativeEffects of Leucosporidium-derived ice-binding protein (LeIBP) on bull semen cryopreservation-
dc.typeArticle-
dc.citation.titleVeterinary Medicine and Science-
dc.citation.number0-
dc.citation.endPage453-
dc.citation.startPage447-
dc.citation.volume6-
dc.contributor.affiliatedAuthorHoon Jang-
dc.contributor.affiliatedAuthorHyo-Jin Kwon-
dc.contributor.affiliatedAuthorJeong Woong Lee-
dc.contributor.alternativeName장훈-
dc.contributor.alternativeName권효진-
dc.contributor.alternativeNameSun-
dc.contributor.alternativeName황성수-
dc.contributor.alternativeName황인-
dc.contributor.alternativeName김성우-
dc.contributor.alternativeName이준-
dc.contributor.alternativeName이성-
dc.contributor.alternativeName이정웅-
dc.identifier.bibliographicCitationVeterinary Medicine and Science, vol. 6, pp. 447-453-
dc.identifier.doi10.1002/vms3.269-
dc.subject.keywordAntioxidant activity-
dc.subject.keywordBull sperm-
dc.subject.keywordCryopreservation-
dc.subject.keywordLeIBP-
dc.subject.localAntioxidant activity-
dc.subject.localBull sperm-
dc.subject.localCryopreservation-
dc.subject.localLeIBP-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.