DC Field | Value | Language |
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dc.contributor.author | Dong-Kyu Kwak | - |
dc.contributor.author | Jin-Sik Kim | - |
dc.contributor.author | Mi-Kyung Lee | - |
dc.contributor.author | K S Ryu | - |
dc.contributor.author | Seung-Wook Chi | - |
dc.date.accessioned | 2020-11-05T13:09:18Z | - |
dc.date.available | 2020-11-05T13:09:18Z | - |
dc.date.issued | 2020 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/23205 | - |
dc.description.abstract | Neuraminidase (NA), one of the major surface glycoproteins of influenza A virus (IAV), is an important diagnostic biomarker and antiviral therapeutic target. Cytolysin A (ClyA) is a nanopore sensor with an internal constriction of 3.3 nm, enabling the detection of protein conformations at the single-molecule level. In this study, a nanopore-based approach is developed for analysis of the enzymatic activity of NA, which facilitates rapid and highly sensitive diagnosis of IAV. Current blockade analysis of the d-glucose/d-galactose-binding protein (GBP) trapped within a type I ClyA-AS (ClyA mutant) nanopore reveals that galactose cleaved from sialyl-galactose by NA of the influenza virus can be detected in real time and at the single-molecule level. Our results show that this nanopore sensor can quantitatively measure the activity of NA with 40?80-fold higher sensitivity than those previously reported. Furthermore, the inhibition of NA is monitored using small-molecule antiviral drugs, such as zanamivir. Taken together, our results reveal that the ClyA protein nanopore can be a valuable platform for the rapid and sensitive point-of-care diagnosis of influenza and for drug screening against the NA target. | - |
dc.publisher | Amer Chem Soc | - |
dc.title | Probing the neuraminidase activity of influenza virus using a cytolysin A protein nanopore | - |
dc.title.alternative | Probing the neuraminidase activity of influenza virus using a cytolysin A protein nanopore | - |
dc.type | Article | - |
dc.citation.title | Analytical Chemistry | - |
dc.citation.number | 21 | - |
dc.citation.endPage | 14308 | - |
dc.citation.startPage | 14303 | - |
dc.citation.volume | 92 | - |
dc.contributor.affiliatedAuthor | Dong-Kyu Kwak | - |
dc.contributor.affiliatedAuthor | Jin-Sik Kim | - |
dc.contributor.affiliatedAuthor | Mi-Kyung Lee | - |
dc.contributor.affiliatedAuthor | Seung-Wook Chi | - |
dc.contributor.alternativeName | 곽동규 | - |
dc.contributor.alternativeName | 김진식 | - |
dc.contributor.alternativeName | 이미경 | - |
dc.contributor.alternativeName | 유경석 | - |
dc.contributor.alternativeName | 지승욱 | - |
dc.identifier.bibliographicCitation | Analytical Chemistry, vol. 92, no. 21, pp. 14303-14308 | - |
dc.identifier.doi | 10.1021/acs.analchem.0c03399 | - |
dc.description.journalClass | Y | - |
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