Probing the neuraminidase activity of influenza virus using a cytolysin A protein nanopore

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dc.contributor.authorDong-Kyu Kwak-
dc.contributor.authorJin-Sik Kim-
dc.contributor.authorMi-Kyung Lee-
dc.contributor.authorK S Ryu-
dc.contributor.authorSeung-Wook Chi-
dc.date.accessioned2020-11-05T13:09:18Z-
dc.date.available2020-11-05T13:09:18Z-
dc.date.issued2020-
dc.identifier.issn0003-2700-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/23205-
dc.description.abstractNeuraminidase (NA), one of the major surface glycoproteins of influenza A virus (IAV), is an important diagnostic biomarker and antiviral therapeutic target. Cytolysin A (ClyA) is a nanopore sensor with an internal constriction of 3.3 nm, enabling the detection of protein conformations at the single-molecule level. In this study, a nanopore-based approach is developed for analysis of the enzymatic activity of NA, which facilitates rapid and highly sensitive diagnosis of IAV. Current blockade analysis of the d-glucose/d-galactose-binding protein (GBP) trapped within a type I ClyA-AS (ClyA mutant) nanopore reveals that galactose cleaved from sialyl-galactose by NA of the influenza virus can be detected in real time and at the single-molecule level. Our results show that this nanopore sensor can quantitatively measure the activity of NA with 40?80-fold higher sensitivity than those previously reported. Furthermore, the inhibition of NA is monitored using small-molecule antiviral drugs, such as zanamivir. Taken together, our results reveal that the ClyA protein nanopore can be a valuable platform for the rapid and sensitive point-of-care diagnosis of influenza and for drug screening against the NA target.-
dc.publisherAmer Chem Soc-
dc.titleProbing the neuraminidase activity of influenza virus using a cytolysin A protein nanopore-
dc.title.alternativeProbing the neuraminidase activity of influenza virus using a cytolysin A protein nanopore-
dc.typeArticle-
dc.citation.titleAnalytical Chemistry-
dc.citation.number21-
dc.citation.endPage14308-
dc.citation.startPage14303-
dc.citation.volume92-
dc.contributor.affiliatedAuthorDong-Kyu Kwak-
dc.contributor.affiliatedAuthorJin-Sik Kim-
dc.contributor.affiliatedAuthorMi-Kyung Lee-
dc.contributor.affiliatedAuthorSeung-Wook Chi-
dc.contributor.alternativeName곽동규-
dc.contributor.alternativeName김진식-
dc.contributor.alternativeName이미경-
dc.contributor.alternativeName유경석-
dc.contributor.alternativeName지승욱-
dc.identifier.bibliographicCitationAnalytical Chemistry, vol. 92, no. 21, pp. 14303-14308-
dc.identifier.doi10.1021/acs.analchem.0c03399-
dc.description.journalClassY-
Appears in Collections:
Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
Division of Biomedical Research > 1. Journal Articles
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