Development of a squaraine-based molecular probe for dual-modal in vivo fluorescence and photoacoustic imaging

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Title
Development of a squaraine-based molecular probe for dual-modal in vivo fluorescence and photoacoustic imaging
Author(s)
Y D Park; Jam-Eon Park; H S Kim; Seung Hyeon Choi; J E Park; J Jeon; Seung Hwan Park
Bibliographic Citation
Bioconjugate Chemistry, vol. 31, no. 11, pp. 2607-2617
Publication Year
2020
Abstract
Dual-modular imaging approaches combining near-infrared (NIR) fluorescence (FLI) and photoacoustic imaging (PAI) require suitable contrast agents to produce dual-modular signals. Although nanoparticles have been used to develop PAI agents, small molecule-based imaging agents have not been extensively studied, highlighting the need to design new fluorophores with an enhanced multifunctional ability. Thus, in this study, we designed a novel squaraine (SQ)-based dye and reported its rational preparation and conjugation with a cancer targeting peptide. Specifically, benzoindole-derived SQ (BSQ) showed strong absorption and fluorescence properties at above 650 nm under aqueous conditions, with a maximum absorption and emission at 665 and 680 nm, respectively. Moreover, PA signal scanning experiments revealed a maximum signal intensity in the range 680-700 nm. BSQ was also conjugated with cyclic arginine-glycine-aspartic acid (cRGD) to improve its active targeting ability for the αvβ3 integrin, which is overexpressed in various cancer and angiogenic cells. A series of in vitro, in vivo, and ex vivo FLI studies showed that the cRGD conjugated BSQ (BSQ-RGD2) successfully stained and targeted αvβ3 integrin-overexpressing tumor cells and xenografts, which were clearly visualized by FLI and PAI. Therefore, BSQ-RGD2 can successfully be applied to dual-modular imaging of the specific biomarker in living animals.
ISSN
1043-1802
Publisher
Amer Chem Soc
DOI
http://dx.doi.org/10.1021/acs.bioconjchem.0c00533
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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