Particulate matter exposure during oocyte maturation: cell cycle arrest, ROS generation, and early apoptosis in mice = 미세먼지 노출이 포유동물 난자성숙에 미치는 영향

Cited 0 time in scopus
Metadata Downloads
Title
Particulate matter exposure during oocyte maturation: cell cycle arrest, ROS generation, and early apoptosis in mice = 미세먼지 노출이 포유동물 난자성숙에 미치는 영향
Author(s)
Yu-Jin JoSeung-Bin YoonByoung Jin ParkSang Il LeeKi Jin KimSe Yong KimMinseong KimJun Ki LeeSang Yong LeeDong Ho LeeTaeho Kwon; Yeonghoon Son; Ja-Rang Lee; Jeongwoo Kwon; Ji-Su Kim
Bibliographic Citation
Frontiers in Cell and Developmental Biology, vol. 8, pp. 602097-602097
Publication Year
2020
Abstract
Particulate matter (PM) is a general atmospheric pollutant released into the air by an anthropogenic and naturally derived mixture of substances. Current studies indicate that fine dust can result in different health defects, including endothelial dysfunction, asthma, lung cancer, cardiovascular diseases, uterine leiomyoma, deterioration in sperm quality, and overall birth impairment. However, the most prominent effects of PM10 (diameter < 10 mM) exposure on the female reproductive system, especially with respect to oocyte maturation, remain unclear. In the present study, maturing mouse oocytes were treated with PM10 and the phenotypes of the resulting toxic effects were investigated. Exposure to PM10 led to impairment of maturation capacity by inducing cell cycle arrest and blocking normal polar body extrusion during in vitro maturation and activation of fertilization of mouse oocytes. Additionally, defects in tubulin formation and DNA alignment were observed in PM10-treated oocytes during metaphase I to anaphase/telophase I transition. Moreover, PM10 induced reactive oxygen species generation, mitochondrial dysfunction, DNA damage, and early apoptosis. Taken together, these results indicate that PM10 exposure leads to a decline in oocyte quality and affects the subsequent embryonic development potential of mammalian oocytes.
Keyword
particulate matteroocyte maturationcell cycle arrestpolar body extrusiontime-lapse microscopy
ISSN
2296-634X
Publisher
Frontiers Media Sa
DOI
http://dx.doi.org/10.3389/fcell.2020.602097
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.