Establishment and characterization of immortalized miniature pig pancreatic cell lines expressing oncogenic K-rasG12D

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Title
Establishment and characterization of immortalized miniature pig pancreatic cell lines expressing oncogenic K-rasG12D
Author(s)
Hae Jun Yang; Bong-Seok SongBo Woong Sim; Yena Jung; Unbin Chae; Dong Gil Lee; Jae Jin Cha; Seo-Jong Baek; Kyung Seob LimWon Seok ChoiHwal Yong LeeHee Chang SonSung Hyun ParkKang Jin JeongPhilyong KangSeung Ho BaekBon-Sang KooHan Na Kim; Yeung Bae Jin; Young-Ho Park; Y K Choo; Sun-Uk Kim
Bibliographic Citation
International Journal of Molecular Sciences, vol. 21, no. 22, pp. 8820-8820
Publication Year
2020
Abstract
In recent decades, many studies on the treatment and prevention of pancreatic cancer have been conducted. However, pancreatic cancer remains incurable, with a high mortality rate. Although mouse models have been widely used for preclinical pancreatic cancer research, these models have many differences from humans. Therefore, large animals may be more useful for the investigation of pancreatic cancer. Pigs have recently emerged as a new model of pancreatic cancer due to their similarities to humans, but no pig pancreatic cancer cell lines have been established for use in drug screening or analysis of tumor biology. Here, we established and characterized an immortalized miniature pig pancreatic cell line derived from primary pancreatic cells and pancreatic cancer-like cells expressing K-rasG12D regulated by the human PTF1A promoter. Using this immortalized cell line, we analyzed the gene expression and phenotypes associated with cancer cell characteristics. Notably, we found that acinar-to-ductal transition was caused by K-rasG12D in the cell line constructed from acinar cells. This may constitute a good research model for the analysis of acinar-to-ductal metaplasia in human pancreatic cancer.
Keyword
pancreatic ductal adenocarcinomaacinar-to-ductal metaplasiaK-rasG12Dminiature pig pancreatic cells
ISSN
1661-6596
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/ijms21228820
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > National Primate Research Center > 1. Journal Articles
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