Development of a rapid selection system for salt-resistant mutants of Nicotiana benthamiana through protoplast culture after gamma irradiation

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Title
Development of a rapid selection system for salt-resistant mutants of Nicotiana benthamiana through protoplast culture after gamma irradiation
Author(s)
Da Mon Jin; Seung Hee Choi; Myoung Hui Lee; Eun Yee Jie; Woo-Seok Ahn; Su Ji Joo; J W Ahn; Y D Jo; S J Ahn; Suk Weon Kim
Bibliographic Citation
Plants-Basel, vol. 9, no. 12, pp. 1720-1720
Publication Year
2020
Abstract
We aimed to develop a novel technology capable of rapidly selecting mutant plant cell lines. Salt resistance was chosen as a rapid selection trait that is easily applicable to protoplast-derived cell colonies. Mesophyll protoplasts were cultured in a medium supplemented with 0, 50, 100, 150, 200, 250, and 300 mM NaCl. At NaCl concentrations ≥ 100 mM, cell colony formation was strongly inhibited after 4 weeks of culture. Tobacco protoplasts irradiated with 0, 50, 100, 200, and 400 Gy were then cultured to investigate the effects of radiation intensity on cell division. The optimal radiation intensity was 50 Gy. To develop salt-resistant tobacco mutant plants, protoplasts irradiated with 50 Gy were cultured in a medium containing 100 mM NaCl. The efficiency of cell colony formation from these protoplasts was approximately 0.002%. A salt-resistant mutant callus was selected and proliferated in the same medium and then transferred to a shoot inducing medium for adventitious shoot formation. The obtained shoots were then cultured in a medium supplemented with 200 mM NaCl and developed into normal plantlets. This rapid selection technology for generating salt-resistant tobacco mutants will be useful for the development of crop varieties resistant to environmental stresses.
Keyword
gammairradiationprotoplast culturerapid selectionsalt-resistantmutantplant regenerationNicotiana benthamiana
ISSN
2223-7747
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/plants9121720
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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