Colorimetric detection of SARS-CoV-2 and drug-resistant pH1N1 using CRISPR/dCas9

Cited 26 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorJeong Moon-
dc.contributor.authorHyung-Jun Kwon-
dc.contributor.authorD Yong-
dc.contributor.authorIn Chul Lee-
dc.contributor.authorHongki Kim-
dc.contributor.authorHyunju Kang-
dc.contributor.authorEun Kyung Lim-
dc.contributor.authorKyu-Sun Lee-
dc.contributor.authorJuyeon Jung-
dc.contributor.authorH G Park-
dc.contributor.authorTaejoon Kang-
dc.date.accessioned2020-12-29T03:30:14Z-
dc.date.available2020-12-29T03:30:14Z-
dc.date.issued2020-
dc.identifier.issn23793694-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/23951-
dc.description.abstractViruses have been a continuous threat to human beings. The coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to a pandemic that is still ongoing worldwide. Previous pandemic influenza A virus (pH1N1) might be re-emerging through a drug-resistant mutation. We report a colorimetric viral detection method based on the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 endonuclease dead (dCas9) system. In this method, RNA in the viral lysate was directly recognized by the CRISPR/dCas9 system with biotin-protospacer adjacent motif (PAM)-presenting oligonucleotide (PAMmer). Streptavidin-horseradish peroxidase then bound to biotin-PAMmer, inducing a color change through the oxidation of 3,3′,5,5′-tetramethylbenzidine. Using the developed method, we successfully identified SARS-CoV-2, pH1N1, and pH1N1/H275Y viruses by the naked eye. Moreover, the detection of viruses in human nasopharyngeal aspirates and sputum was demonstrated. Finally, clinical samples from COVID-19 patients led to a successful diagnosis. We anticipate that the current method can be employed for simple and accurate diagnosis of viruses.-
dc.publisherAmer Chem Soc-
dc.titleColorimetric detection of SARS-CoV-2 and drug-resistant pH1N1 using CRISPR/dCas9-
dc.title.alternativeColorimetric detection of SARS-CoV-2 and drug-resistant pH1N1 using CRISPR/dCas9-
dc.typeArticle-
dc.citation.titleACS Sensors-
dc.citation.number0-
dc.citation.endPage4026-
dc.citation.startPage4017-
dc.citation.volume5-
dc.contributor.affiliatedAuthorJeong Moon-
dc.contributor.affiliatedAuthorHyung-Jun Kwon-
dc.contributor.affiliatedAuthorIn Chul Lee-
dc.contributor.affiliatedAuthorHongki Kim-
dc.contributor.affiliatedAuthorHyunju Kang-
dc.contributor.affiliatedAuthorEun Kyung Lim-
dc.contributor.affiliatedAuthorKyu-Sun Lee-
dc.contributor.affiliatedAuthorJuyeon Jung-
dc.contributor.affiliatedAuthorTaejoon Kang-
dc.contributor.alternativeName문정-
dc.contributor.alternativeName권형준-
dc.contributor.alternativeName용동은-
dc.contributor.alternativeName이인철-
dc.contributor.alternativeName김홍기-
dc.contributor.alternativeName강현주-
dc.contributor.alternativeName임은경-
dc.contributor.alternativeName이규선-
dc.contributor.alternativeName정주연-
dc.contributor.alternativeName박현규-
dc.contributor.alternativeName강태준-
dc.identifier.bibliographicCitationACS Sensors, vol. 5, pp. 4017-4026-
dc.identifier.doi10.1021/acssensors.0c01929-
dc.subject.keywordSARS-CoV-2-
dc.subject.keywordCOVID-19-
dc.subject.keywordDrug-resistance-
dc.subject.keywordInfluenza virus-
dc.subject.keywordCRISPR/dCas9-
dc.subject.keywordColorimetry-
dc.subject.localSARS-CoV-2-
dc.subject.localCOVID-19-
dc.subject.localDrug-resistance-
dc.subject.localInfluenza virus-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.