Salmonella vaccine vector system for foot-and-mouth disease virus and evaluation of its efficacy with virus-like particles = 구제역 바이러스에 대한 살모넬라 백신 백터 시스템과 바이러스 유사입자에 대한 백신 효능 평가

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dc.contributor.authorY Zhi-
dc.contributor.authorH J Ji-
dc.contributor.authorH Guo-
dc.contributor.authorJ H Lim-
dc.contributor.authorE B Byun-
dc.contributor.authorWoo Sik Kim-
dc.contributor.authorH S Seo-
dc.date.accessioned2021-01-07T03:31:28Z-
dc.date.available2021-01-07T03:31:28Z-
dc.date.issued2021-
dc.identifier.issn2076-393X-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/23974-
dc.description.abstractFoot-and-mouth disease virus (FMDV) causes a highly contagious and devastating disease in livestock animals and has a great potential to cause severe economic loss worldwide. The major antigen of FMDV capsid protein, VP1, contains the major B-cell epitope responsible for effectively eliciting protective humoral immunity. In this study, irradiated Salmonella Typhimurium (KST0666) were used as transgenic vectors containing stress-inducible plasmid pRECN-VP1 to deliver the VP1 protein from FMDV-type A/WH/CHA/09. Mice were orally inoculated with ATOMASal-L3 harboring pRECN-VP1, and FMDV virus-like particles, where (VLPFMDV)-specific humoral, mucosal, and cellular immune responses were evaluated. Mice vaccinated with attenuated Salmonella (KST0666) expressing VP1 (named KST0669) showed high levels of VLP-specific IgA in feces and IgG in serum, with high FMDV neutralization titer. Moreover, KST0669-vaccinated mice showed increased population of IFN-γ (type 1 T helper cells; Th1 cells)-, IL-5 (Th2 cells)-, and IL-17A (Th17 cells)-expressing CD4+ as well as activated CD8+ T cells (IFN-γ+CD8+ cells), detected by stimulating VLPFMDV. All data indicate that our Salmonella vector system successfully delivered FMDV VP1 to immune cells and that the humoral and cellular efficacy of the vaccine can be easily evaluated using VLPFMDV in a Biosafety Level I (BSL1) laboratory.-
dc.publisherMDPI-
dc.titleSalmonella vaccine vector system for foot-and-mouth disease virus and evaluation of its efficacy with virus-like particles = 구제역 바이러스에 대한 살모넬라 백신 백터 시스템과 바이러스 유사입자에 대한 백신 효능 평가-
dc.title.alternativeSalmonella vaccine vector system for foot-and-mouth disease virus and evaluation of its efficacy with virus-like particles-
dc.typeArticle-
dc.citation.titleVaccines-
dc.citation.number1-
dc.citation.endPage22-
dc.citation.startPage22-
dc.citation.volume9-
dc.contributor.affiliatedAuthorWoo Sik Kim-
dc.contributor.alternativeNameZhi-
dc.contributor.alternativeName지현정-
dc.contributor.alternativeNameGuo-
dc.contributor.alternativeName임재향-
dc.contributor.alternativeName변의백-
dc.contributor.alternativeName김우식-
dc.contributor.alternativeName서호성-
dc.identifier.bibliographicCitationVaccines, vol. 9, no. 1, pp. 22-22-
dc.identifier.doi10.3390/vaccines9010022-
dc.subject.keywordFoot-and-mouth disease-
dc.subject.keywordVP1-
dc.subject.keywordLive attenuated Salmonella vector-
dc.subject.keywordMucosal immunity-
dc.subject.keywordVirus-like particle-
dc.subject.keywordRadiation mutation-
dc.subject.localfoot-and-mouth disease-
dc.subject.localFoot-and-Mouth Disease-
dc.subject.localFoot-and-mouth disease-
dc.subject.localVP1-
dc.subject.localLive attenuated Salmonella vector-
dc.subject.localMucosal immunity-
dc.subject.localmucosal immunity-
dc.subject.localvirus-like praticle-
dc.subject.localvirus-like particles-
dc.subject.localVirus-like particle-
dc.subject.localvirus-like particle-
dc.subject.localRadiation mutation-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
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