Overexpression of the golden SNP-carrying Orange gene enhances carotenoid accumulation and heat stress tolerance in sweetpotato plants

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dc.contributor.authorSo-Eun Kim-
dc.contributor.authorChan-Ju Lee-
dc.contributor.authorSulu Park-
dc.contributor.authorYe-Hoon Lim-
dc.contributor.authorW S Park-
dc.contributor.authorH J Kim-
dc.contributor.authorM J Ahn-
dc.contributor.authorSang Soo Kwak-
dc.contributor.authorHo Soo Kim-
dc.date.accessioned2021-01-07T03:31:40Z-
dc.date.available2021-01-07T03:31:40Z-
dc.date.issued2021-
dc.identifier.issn2076-3921-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/23978-
dc.description.abstractCarotenoids function as photosynthetic accessory pigments, antioxidants, and vitamin A precursors. We recently showed that transgenic sweetpotato calli overexpressing the mutant sweetpotato (Ipomoea batatas [L.] Lam) Orange gene (IbOr-R96H), which carries a single nucleotide polymorphism responsible for Arg to His substitution at amino acid position 96, exhibited dramatically higher carotenoid content and abiotic stress tolerance than calli overexpressing the wild-type IbOr gene (IbOr-WT). In this study, we generated transgenic sweetpotato plants overexpressing IbOr-R96H under the control of the cauliflower mosaic virus (CaMV) 35S promoter via Agrobacterium-mediated transformation. The total carotenoid contents of IbOr-R96H storage roots (light-orange flesh) and IbOr-WT storage roots (light-yellow flesh) were 5.4?19.6 and 3.2-fold higher, respectively, than those of non-transgenic (NT) storage roots (white flesh). The β-carotene content of IbOr-R96H storage roots was up to 186.2-fold higher than that of NT storage roots. In addition, IbOr-R96H plants showed greater tolerance to heat stress (47 °C) than NT and IbOr-WT plants, possibly because of higher DPPH radical scavenging activity and ABA contents. These results indicate that IbOr-R96H is a promising strategy for developing new sweetpotato cultivars with improved carotenoid contents and heat stress tolerance.-
dc.publisherMDPI-
dc.titleOverexpression of the golden SNP-carrying Orange gene enhances carotenoid accumulation and heat stress tolerance in sweetpotato plants-
dc.title.alternativeOverexpression of the golden SNP-carrying Orange gene enhances carotenoid accumulation and heat stress tolerance in sweetpotato plants-
dc.typeArticle-
dc.citation.titleAntioxidants-
dc.citation.number1-
dc.citation.endPage51-
dc.citation.startPage51-
dc.citation.volume10-
dc.contributor.affiliatedAuthorSo-Eun Kim-
dc.contributor.affiliatedAuthorChan-Ju Lee-
dc.contributor.affiliatedAuthorSulu Park-
dc.contributor.affiliatedAuthorYe-Hoon Lim-
dc.contributor.affiliatedAuthorSang Soo Kwak-
dc.contributor.affiliatedAuthorHo Soo Kim-
dc.contributor.alternativeName김소은-
dc.contributor.alternativeName이찬주-
dc.contributor.alternativeName박슬우-
dc.contributor.alternativeName임예훈-
dc.contributor.alternativeName박우성-
dc.contributor.alternativeName김혜진-
dc.contributor.alternativeName안미정-
dc.contributor.alternativeName곽상수-
dc.contributor.alternativeName김호수-
dc.identifier.bibliographicCitationAntioxidants, vol. 10, no. 1, pp. 51-51-
dc.identifier.doi10.3390/antiox10010051-
dc.subject.keywordSweetpotato-
dc.subject.keywordCarotenoid-
dc.subject.keywordOrange-
dc.subject.keywordIbOr-
dc.subject.keywordIbOr-R96H-
dc.subject.localsweet potato-
dc.subject.localsweet potatoes-
dc.subject.localSweet potato (Ipomoea batatas L. Lam)-
dc.subject.localSweet potato (Ipomoea batatas)-
dc.subject.localSweet otato-
dc.subject.localsweet potato (Ipomoea batatas)-
dc.subject.localipomoea batatas-
dc.subject.localSweetpotato (Ipomoea batatas)-
dc.subject.localsweetpotato-
dc.subject.localSweetpotato Ipomoea batatas-
dc.subject.localSweetpotato (Ipomoea batatas L.)-
dc.subject.localSweetpotato-
dc.subject.localSweetpotato (Ipomoea batatas (L.) Lam)-
dc.subject.localSweet potato-
dc.subject.localIpomoea batatas-
dc.subject.localSweet potato (Ipomoea batatas (L.) Lam.)-
dc.subject.localCarotenoids-
dc.subject.localcarotenoids-
dc.subject.localcarotenoid-
dc.subject.localCarotenoid-
dc.subject.localorange-
dc.subject.localOrange-
dc.subject.localIbOr-
dc.subject.localIbOr-R96H-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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