Process optimization for mass production of 2,3-butanediol by Bacillus subtilis CS13

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Title
Process optimization for mass production of 2,3-butanediol by Bacillus subtilis CS13
Author(s)
D Wang; Baek Rock OhS LeeDo-hyoung Kim; M H Joe
Bibliographic Citation
Biotechnology for Biofuels, vol. 14, pp. 15-15
Publication Year
2021
Abstract
Background: Bacillus subtilis CS13 was previously isolated for 2,3-butanediol (2,3-BD) and poly-γ-glutamic acid (γ-PGA) co-production. When culturing this strain without L-glutamic acid in the medium, 2,3-BD is the main metabolic product. 2,3-BD is an important substance and fuel with applications in the chemical, food, and pharmaceutical industries. However, the yield and productivity for the B. subtilis strain should be improved for more efficient production of 2,3-BD. Results: The medium composition, which contained 281.1 g/L sucrose, 21.9 g/L ammonium citrate, and 3.6 g/L MgSO4·7H2O, was optimized by response surface methodology for 2,3-BD production using B. subtilis CS13. The maximum amount of 2,3-BD (125.5±3.1 g/L) was obtained from the optimized medium after 96 h. The highest concentration and productivity of 2,3-BD were achieved simultaneously at an agitation speed of 500 rpm and aeration rate of 2 L/min in the batch cultures. A total of 132.4±4.4 g/L 2,3-BD was obtained with a productivity of 2.45±0.08 g/L/h and yield of 0.45 g2,3-BD/gsucrose by fed-batch fermentation. The meso-2,3-BD/2,3-BD ratio of the 2,3-BD produced by B. subtilis CS13 was 92.1%. Furthermore, 89.6±2.8 g/L 2,3-BD with a productivity of 2.13±0.07 g/L/h and yield of 0.42 g2,3-BD/gsugar was achieved using molasses as a carbon source. Conclusions: The production of 2,3-BD by B. subtilis CS13 showed a higher concentration, productivity, and yield compared to the reported generally recognized as safe 2,3-BD producers. These results suggest that B. subtilis CS13 is a promising strain for industrial-scale production of 2,3-BD.
Keyword
2,3-butanediolB. subtilis CS13Response surface methodologyFermentation
ISSN
1754-6834
Publisher
Springer-BMC
DOI
http://dx.doi.org/10.1186/s13068-020-01859-w
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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