A therapeutic neutralizing antibody targeting receptor binding domain of SARS-CoV-2 spike protein

Cited 31 time in scopus
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Title
A therapeutic neutralizing antibody targeting receptor binding domain of SARS-CoV-2 spike protein
Author(s)
C Kim; D K Ryu; J Lee; Y I Kim; J M Seo; Y G Kim; J H Jeong; M Kim; J I Kim; P Kim; J S Bae; E Y Shim; M S Lee; M S Kim; H Noh; G S Park; J S Park; D Son; Y An; J N Lee; Hanseul OhBon-Sang KooJung Joo HongChoong-Min Ryu; Y K Choi; S Y Lee
Bibliographic Citation
Nature Communications, vol. 12, pp. 288-288
Publication Year
2021
Abstract
Vaccines and therapeutics are urgently needed for the pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we screen human monoclonal antibodies (mAb) targeting the receptor binding domain (RBD) of the viral spike protein via antibody library constructed from peripheral blood mononuclear cells of a convalescent patient. The CT-P59 mAb potently neutralizes SARS-CoV-2 isolates including the D614G variant without antibody-dependent enhancement effect. Complex crystal structure of CT-P59 Fab/RBD shows that CT-P59 blocks interaction regions of RBD for angiotensin converting enzyme 2 (ACE2) receptor with an orientation that is notably different from previously reported RBD-targeting mAbs. Furthermore, therapeutic effects of CT-P59 are evaluated in three animal models (ferret, hamster, and rhesus monkey), demonstrating a substantial reduction in viral titer along with alleviation of clinical symptoms. Therefore, CT-P59 may be a promising therapeutic candidate for COVID-19.
ISSN
2041-1723
Publisher
Springer-Nature Pub Group
DOI
http://dx.doi.org/10.1038/s41467-020-20602-5
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > National Primate Research Center > 1. Journal Articles
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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