Antagonistic control of genetic circuit performance for rapid analysis of targeted enzyme activity in living cells = 유전자회로의 길항적 제어를 이용한 세포 내 표적 효소 활성의 신속한 분석

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dc.contributor.authorKil Koang Kwon-
dc.contributor.authorHaseong Kim-
dc.contributor.authorSoo Jin Yeom-
dc.contributor.authorEugene Rha-
dc.contributor.authorJinju Lee-
dc.contributor.authorHyewon Lee-
dc.contributor.authorDae Hee Lee-
dc.contributor.authorSeung Goo Lee-
dc.date.accessioned2021-01-20T03:30:31Z-
dc.date.available2021-01-20T03:30:31Z-
dc.date.issued2021-
dc.identifier.issn2296889X-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/24019-
dc.description.abstractGenetic circuits have been developed for quantitative measurement of enzyme activity, metabolic engineering of strain development, and dynamic regulation of microbial cells. A genetic circuit consists of several bio-elements, including enzymes and regulatory cassettes, that can generate the desired output signal, which is then used as a precise criterion for enzyme screening and engineering. Antagonists and inhibitors are small molecules with inhibitory effects on regulators and enzymes, respectively. In this study, an antagonist and an inhibitor were applied to a genetic circuit for a dynamic detection range. We developed a genetic circuit relying on regulators and enzymes, allowing for straightforward control of its output signal without additional genetic modification. We used para-nitrophenol and alanine as an antagonist of DmpR and inhibitor of tyrosine phenol-lyase, respectively. We show that the antagonist resets the detection range of the genetic circuit similarly to a resistor in an electrical logic circuit. These biological resistors in genetic circuits can be used as a rapid and precise controller of variable outputs with minimal circuit configuration.-
dc.publisherFrontiers Media Sa-
dc.titleAntagonistic control of genetic circuit performance for rapid analysis of targeted enzyme activity in living cells = 유전자회로의 길항적 제어를 이용한 세포 내 표적 효소 활성의 신속한 분석-
dc.title.alternativeAntagonistic control of genetic circuit performance for rapid analysis of targeted enzyme activity in living cells-
dc.typeArticle-
dc.citation.titleFrontiers in Molecular Biosciences-
dc.citation.number0-
dc.citation.endPage599878-
dc.citation.startPage599878-
dc.citation.volume7-
dc.contributor.affiliatedAuthorKil Koang Kwon-
dc.contributor.affiliatedAuthorHaseong Kim-
dc.contributor.affiliatedAuthorSoo Jin Yeom-
dc.contributor.affiliatedAuthorEugene Rha-
dc.contributor.affiliatedAuthorJinju Lee-
dc.contributor.affiliatedAuthorHyewon Lee-
dc.contributor.affiliatedAuthorDae Hee Lee-
dc.contributor.affiliatedAuthorSeung Goo Lee-
dc.contributor.alternativeName권길광-
dc.contributor.alternativeName김하성-
dc.contributor.alternativeName염수진-
dc.contributor.alternativeName나유진-
dc.contributor.alternativeName이진주-
dc.contributor.alternativeName이혜원-
dc.contributor.alternativeName이대희-
dc.contributor.alternativeName이승구-
dc.identifier.bibliographicCitationFrontiers in Molecular Biosciences, vol. 7, pp. 599878-599878-
dc.identifier.doi10.3389/fmolb.2020.599878-
dc.subject.keywordInhibitor-
dc.subject.keywordAntagonist-
dc.subject.keywordGenetic circuit-
dc.subject.keywordPhenolic compound-
dc.subject.keywordFlow cytometry-
dc.subject.keywordResistor-
dc.subject.localInhibitor-
dc.subject.localGenetic circuit-
dc.subject.localPhenolic compound-
dc.subject.localFlow cytometry-
dc.description.journalClassY-
Appears in Collections:
Division of Biomaterials Research > 1. Journal Articles
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
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