Development of a miRNA-controlled dual-sensing system and its application for targeting miR-21 signaling in tumorigenesis

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dc.contributor.authorY Seo-
dc.contributor.authorS S Kim-
dc.contributor.authorN Kim-
dc.contributor.authorSungchan Cho-
dc.contributor.authorJ B Park-
dc.contributor.authorJ H Kim-
dc.date.accessioned2021-01-22T03:30:47Z-
dc.date.available2021-01-22T03:30:47Z-
dc.date.issued2020-
dc.identifier.issn1226-3613-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/24023-
dc.description.abstractMicroRNAs (miRNAs) are considered to be strong prognostic markers and key therapeutic targets in human diseases, especially cancer. A sensitive monitoring platform for cancer-associated miRNA (oncomiR) action is needed for mechanistic studies, preclinical evaluation, and inhibitor screening. In this study, we developed and systemically applied a sensitive and efficient lentivirus-based system for monitoring oncomiR actions, essentially miR-21. The specificity and sensitivity of "miRDREL" against various oncomiRs were validated by checking for tight correlations between their expression and targeting efficacy. Experiments based on the transfection of synthetic mimics and antagomir-mediated depletion of oncomiRs further confirmed the specificity of the system. Systemic application of miRDRELs to natural oncomiR targets, knockdown of key microprocessors, and physiological triggering of oncomiRs also demonstrated that the system is an effective tool for monitoring cellular oncomiR action. Importantly, molecular modeling-based screening confirmed the action of the miR-21-targeting drug ivermectin and led to the identification of a new effective derivative, GW4064, for inhibiting oncogenic DDX23-miR-21 signaling. Furthermore, proteomic-kinase inhibitor screenings identified a novel oncogenic kinome-DDX23-miR-21 axis and thus expands our understanding of miR-21 targeting therapeutics in tumorigenesis. Taken together, these data indicate that miRDREL and its versatile application have great potential in basic, preclinical studies and drug development pipelines for miRNA-related diseases, especially cancer.-
dc.publisherSpringer-Nature Pub Group-
dc.titleDevelopment of a miRNA-controlled dual-sensing system and its application for targeting miR-21 signaling in tumorigenesis-
dc.title.alternativeDevelopment of a miRNA-controlled dual-sensing system and its application for targeting miR-21 signaling in tumorigenesis-
dc.typeArticle-
dc.citation.titleExperimental and Molecular Medicine-
dc.citation.number12-
dc.citation.endPage2004-
dc.citation.startPage1989-
dc.citation.volume52-
dc.contributor.affiliatedAuthorSungchan Cho-
dc.contributor.alternativeName서윤아-
dc.contributor.alternativeName김성수-
dc.contributor.alternativeName김남두-
dc.contributor.alternativeName조성찬-
dc.contributor.alternativeName박종배-
dc.contributor.alternativeName김종헌-
dc.identifier.bibliographicCitationExperimental and Molecular Medicine, vol. 52, no. 12, pp. 1989-2004-
dc.identifier.doi10.1038/s12276-020-00537-z-
dc.description.journalClassY-
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Ochang Branch Institute > Nucleic Acid Therapeutics Research Center > 1. Journal Articles
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