Potential anti-wrinkle activity of Chlorella sp. HS1-derived oil components on human dermal fibroblasts = 인간 진피섬유아세포에서 Chlorella sp. HS1 유래 오일의 콜라겐 발현 증가 효력에 관한 연구

Abstract

Purpose: In this study, we investigated the effects of Chlorella sp. HS1-derived oils on collagen expressions in dermal fibroblasts. Methods: We prepared the oil extracts from Chlorella sp . HS1 biomass, and high-performance liquid chromatography analysis was used in the identification and quantitation of components in the oil extracts. Using human dermal fibroblasts, cell viability and quantitative RT-PCR (qRTPCR)-mediated expression analysis of collagen and matrix metalloprotease (MMP ) genes after oil treatments. We further investigated whether the effect of the oil extracts on collagen expression was related to the major component, β-carotene, of the extracts. In addition, we analyzed the effect of photoprotective effect of β-carotene on ultraviolet (UV)-irradiated dermal fibroblasts. Results: We found that under 50 μg/mL of the oil extracts had little cytotoxicity in skin cells including epidermal keratinocytes, melanocytes and dermal fibroblasts. The HPLC analysis revealed that β-carotene was identified as the major non-polar molecules. Next, we found that the oil-derived β-carotene was little cytotoxicity in dermal fibroblasts. Further experiments revealed that treatment with the oil extracts significantly upregulated collagen type 1 and 3 mRNAs and downregulated MMP1 and MMP3 mRNAs in dermal fibroblasts. Also, we found that these effects were showed in β-carotene-treated cells. In addition, β-carotene significantly attenuated UVA-induced downregulation of collagen type 1 and 3 expressions. Conclusion: Therefore, our results suggested that the oil extracts had a potential anti-wrinkle agent via its major component, β-carotene.