Evaluation of the acute toxicity of theoredoxin (TRX) transgenic soybean to Daphnia magna

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dc.contributor.authorS D Oh-
dc.contributor.authorS K Min-
dc.contributor.authorJ K Kim-
dc.contributor.authorJung-Ho Park-
dc.contributor.authorChang-Gi Kim-
dc.contributor.authorS Y Park-
dc.date.accessioned2021-01-30T03:30:25Z-
dc.date.available2021-01-30T03:30:25Z-
dc.date.issued2020-
dc.identifier.issn24662402-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/24051-
dc.description.abstractTheoredoxin (TRX) transgenic soybeans were developed using the human Theoredoxin gene under the control of the β-conglycinin promoter with a selection marker, the phosphinothricin acetyltransferase (PAT) gene. This study was done to assess the acute toxicity of a genetically modified (GM) soybean using the fresh water planktonic crustacean Daphnia magna. The acute toxicity effect of the TRX soybean and non-GM soybean (Gwangan) on D. magna was investigated at different concentrations (0, 156, 313, 625, 1,250, 2,500, and 5,000 mg·L-1). The TRX soybean used for the test was confirmed to express the TRX/PAT genes by PCR and enzyme-linked immunosorbent assay (ELISA). D. magna feeding tests showed no significant differences in the cumulative immobility or an abnormal response with either the TRX soybean or non-GM soybean. The feeding study showed a similar abnormal response and cumulative immobility of the D. magna between the TRX soybean and Gwangan treatments. Additionally, the 48 h-EC50 values for the TRX and Gwangan soybeans were 755.6 and 778 mg·L-1, respectively. The soybean NOEC (no observed effect concentration) value for D. magna was suggested to be 156 mg·L-1. These results suggest that there is no significant difference in toxicity to Daphnia magna between the TRX soybean and its non-GM counterpart.-
dc.titleEvaluation of the acute toxicity of theoredoxin (TRX) transgenic soybean to Daphnia magna-
dc.title.alternativeEvaluation of the acute toxicity of theoredoxin (TRX) transgenic soybean to Daphnia magna-
dc.typeArticle-
dc.citation.titleKorean Journal of Agricultural Science-
dc.citation.number4-
dc.citation.endPage802-
dc.citation.startPage791-
dc.citation.volume47-
dc.contributor.affiliatedAuthorJung-Ho Park-
dc.contributor.affiliatedAuthorChang-Gi Kim-
dc.contributor.alternativeName오성덕-
dc.contributor.alternativeName민석기-
dc.contributor.alternativeName김재광-
dc.contributor.alternativeName박정호-
dc.contributor.alternativeName김창기-
dc.contributor.alternativeName박수연-
dc.identifier.bibliographicCitationKorean Journal of Agricultural Science, vol. 47, no. 4, pp. 791-802-
dc.identifier.doi10.7744/kjoas.20200066-
dc.subject.keywordDaphnia magna-
dc.subject.keywordEnvironment risk-
dc.subject.keywordTheoredoxin soybean-
dc.description.journalClassN-
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Ochang Branch Institute > Division of Bioinfrastructure > Bio-Evaluation Center > 1. Journal Articles
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