DC Field | Value | Language |
---|---|---|
dc.contributor.author | Su Ui Lee | - |
dc.contributor.author | Mun-Ock Kim | - |
dc.contributor.author | Myung Ji Kang | - |
dc.contributor.author | Eun Sol Oh | - |
dc.contributor.author | H Ro | - |
dc.contributor.author | Ro-Woon Lee | - |
dc.contributor.author | Yu Na Song | - |
dc.contributor.author | Sunin Jung | - |
dc.contributor.author | Jae-Won Lee | - |
dc.contributor.author | Soo Yun Lee | - |
dc.contributor.author | Taeyeol Bae | - |
dc.contributor.author | S T Hong | - |
dc.contributor.author | Tae-Don Kim | - |
dc.date.accessioned | 2021-02-03T03:30:30Z | - |
dc.date.available | 2021-02-03T03:30:30Z | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/24054 | - |
dc.description.abstract | Airway mucus secretion is an essential innate immune response for host protection. However, overproduction and hypersecretion of mucus, mainly composed of the gel- forming MUC5AC protein, are significant risk factors for patients with asthma and chronic obstructive pulmonary disease (COPD). The transforming growth factor β (TGFβ) signaling pathway negatively regulates MUC5AC expression; however, the underlying molecular mechanism is not fully understood. Here, we showed that TGFβ significantly reduces the expression of MUC5AC mRNA and its protein in NCI-H292 cells, a human mucoepidermoid carcinoma cell line. This reduced MUC5AC expression was restored by a TGFβ receptor inhibitor (SB431542), but not by the inhibition of NF-κB (BAY11-7082 or Triptolide) or PI3K (LY294002) activities. TGFβ-activated Smad3 dose-dependently bound to MUC5AC promoter. Notably, TGFβ-activated Smad3 recruited HDAC2 and facilitated nuclear translocation of HDAC2, thereby inducing the deacetylation of NF-κB at K310, which is essential for a reduction in NF-κB transcriptional activity. Both TGFβ-induced nuclear translocation of Smad3/HDAC2 and deacetylation of NF-κB at K310 were suppressed by a Smad3 inhibitor (SIS3). These results suggest that the TGFβ-activated Smad3/HDAC2 complex is an essential negative regulator for MUC5AC expression and an epigenetic regulator for NF-κB acetylation. Therefore, these results collectively suggest that modulation of the TGFβ1/Smad3/HDAC2/NF-κB pathway axis can be a promising way to improve lung function as a treatment strategy for asthma and COPD. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Transforming growth factor β inhibits MUC5AC expression by Smad3/HDAC2 complex formation and NF-κB deacetylation at K310 in NCI-H292 cells | - |
dc.title.alternative | Transforming growth factor β inhibits MUC5AC expression by Smad3/HDAC2 complex formation and NF-κB deacetylation at K310 in NCI-H292 cells | - |
dc.type | Article | - |
dc.citation.title | Molecules and Cells | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 49 | - |
dc.citation.startPage | 38 | - |
dc.citation.volume | 44 | - |
dc.contributor.affiliatedAuthor | Su Ui Lee | - |
dc.contributor.affiliatedAuthor | Mun-Ock Kim | - |
dc.contributor.affiliatedAuthor | Myung Ji Kang | - |
dc.contributor.affiliatedAuthor | Eun Sol Oh | - |
dc.contributor.affiliatedAuthor | Ro-Woon Lee | - |
dc.contributor.affiliatedAuthor | Yu Na Song | - |
dc.contributor.affiliatedAuthor | Sunin Jung | - |
dc.contributor.affiliatedAuthor | Jae-Won Lee | - |
dc.contributor.affiliatedAuthor | Soo Yun Lee | - |
dc.contributor.affiliatedAuthor | Taeyeol Bae | - |
dc.contributor.affiliatedAuthor | Tae-Don Kim | - |
dc.contributor.alternativeName | 이수의 | - |
dc.contributor.alternativeName | 김문옥 | - |
dc.contributor.alternativeName | 강명지 | - |
dc.contributor.alternativeName | 오은솔 | - |
dc.contributor.alternativeName | 노현주 | - |
dc.contributor.alternativeName | 이로운 | - |
dc.contributor.alternativeName | 송유나 | - |
dc.contributor.alternativeName | 정선인 | - |
dc.contributor.alternativeName | 이재원 | - |
dc.contributor.alternativeName | 이수연 | - |
dc.contributor.alternativeName | 배태열 | - |
dc.contributor.alternativeName | 홍성태 | - |
dc.contributor.alternativeName | 김태돈 | - |
dc.identifier.bibliographicCitation | Molecules and Cells, vol. 44, no. 1, pp. 38-49 | - |
dc.identifier.doi | 10.14348/molcells.2020.0188 | - |
dc.subject.keyword | HDAC2 | - |
dc.subject.keyword | MUC5AC | - |
dc.subject.keyword | NF-κB | - |
dc.subject.keyword | Smad3 | - |
dc.subject.keyword | Transforming growth factor β | - |
dc.subject.local | HDAC2 | - |
dc.subject.local | MUC5AC | - |
dc.subject.local | NFkappaB | - |
dc.subject.local | NFκB | - |
dc.subject.local | Nf-κB | - |
dc.subject.local | Nf-κb | - |
dc.subject.local | Nuclear factor (NF)-κB | - |
dc.subject.local | Nuclear factor kappa B | - |
dc.subject.local | Nuclear factor kappaB | - |
dc.subject.local | Nuclear factor κB | - |
dc.subject.local | Nuclear factor κB (NF-κB) | - |
dc.subject.local | Nuclear factor-kappa B | - |
dc.subject.local | Nuclear factor-kappa B (NF-κB) | - |
dc.subject.local | Nuclear factor-kappaB | - |
dc.subject.local | Nuclear factor-κB | - |
dc.subject.local | Nuclear factor-κb | - |
dc.subject.local | NF-kB | - |
dc.subject.local | NF-kappa B | - |
dc.subject.local | NF-kappaB | - |
dc.subject.local | NF-ΚB | - |
dc.subject.local | NF-κ B | - |
dc.subject.local | NF-κB | - |
dc.subject.local | NF-κB (nuclear factor kappa-B) | - |
dc.subject.local | nuclear factor kappa B | - |
dc.subject.local | nuclear factor κB | - |
dc.subject.local | nuclear factor-kappaB | - |
dc.subject.local | nuclear factor-kappaB (NF-κB) | - |
dc.subject.local | nuclear factor-κB | - |
dc.subject.local | nuclear factorκB | - |
dc.subject.local | Smad3 | - |
dc.subject.local | Transforming growth factor beta | - |
dc.subject.local | Transforming growth factor-β | - |
dc.subject.local | Transforming growth factor β | - |
dc.description.journalClass | Y | - |
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