Lycopene improves in vitro development of porcine embryos by reducing oxidative stress and apoptosis

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dc.contributor.authorHyo-Gu Kang-
dc.contributor.authorSanghoon Lee-
dc.contributor.authorPil-Soo Jeong-
dc.contributor.authorMin Ju Kim-
dc.contributor.authorSoo-Hyun Park-
dc.contributor.authorYe Eun Joo-
dc.contributor.authorSung Hyun Park-
dc.contributor.authorBong-Seok Song-
dc.contributor.authorSun-Uk Kim-
dc.contributor.authorM K Kim-
dc.contributor.authorBo Woong Sim-
dc.date.accessioned2021-02-09T03:30:28Z-
dc.date.available2021-02-09T03:30:28Z-
dc.date.issued2021-
dc.identifier.issn20763921-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/24070-
dc.description.abstractIn vitro culture (IVC) for porcine embryo development is inferior compared to in vivo development because oxidative stress can be induced by the production of excessive reactive oxygen species (ROS) under high oxygen tension in the in vitro environment. To overcome this problem, we investigated the effect of lycopene, an antioxidant carotenoid, on developmental competence and the mechanisms involved in mitochondria-dependent apoptosis pathways in porcine embryos. In vitro fertilized (IVF) embryos were cultured in IVC medium supplemented with 0, 0.02, 0.05, 0.1, or 0.2 μM lycopene. The results indicate that 0.1 μM lycopene significantly increased the rate of blastocyst formation and the total cell numbers, including trophectoderm cell numbers, on Day In terms of mitochondria-dependent apoptosis, IVF embryos treated with 0.1 μM lycopene exhibited significantly decreased levels of ROS, increased mitochondrial membrane potential, and decreased expression of cytochrome c on Days 2 and Furthermore, 0.1 μM lycopene significantly decreased the number and percentage of caspase 3-positive and apoptotic cells in Day-6 blastocysts. In addition, Day-2 embryos and Day-6 blastocysts treated with 0.1 μM lycopene showed significantly reduced mRNA expression related to antioxidant enzymes (SOD1, SOD2, CATALASE) and apoptosis (BAX/BCL2L1 ratio). These results indicate that lycopene supplementation during the entire period of IVC enhanced embryonic development in pigs by regulating oxidative stress and mitochondria-dependent apoptosis.-
dc.publisherMDPI-
dc.titleLycopene improves in vitro development of porcine embryos by reducing oxidative stress and apoptosis-
dc.title.alternativeLycopene improves in vitro development of porcine embryos by reducing oxidative stress and apoptosis-
dc.typeArticle-
dc.citation.titleAntioxidants-
dc.citation.number2-
dc.citation.endPage230-
dc.citation.startPage230-
dc.citation.volume10-
dc.contributor.affiliatedAuthorHyo-Gu Kang-
dc.contributor.affiliatedAuthorSanghoon Lee-
dc.contributor.affiliatedAuthorPil-Soo Jeong-
dc.contributor.affiliatedAuthorMin Ju Kim-
dc.contributor.affiliatedAuthorSoo-Hyun Park-
dc.contributor.affiliatedAuthorYe Eun Joo-
dc.contributor.affiliatedAuthorSung Hyun Park-
dc.contributor.affiliatedAuthorBong-Seok Song-
dc.contributor.affiliatedAuthorSun-Uk Kim-
dc.contributor.affiliatedAuthorBo Woong Sim-
dc.contributor.alternativeName강효구-
dc.contributor.alternativeName이상훈-
dc.contributor.alternativeName정필수-
dc.contributor.alternativeName김민주-
dc.contributor.alternativeName박수현-
dc.contributor.alternativeName주예은-
dc.contributor.alternativeName박성현-
dc.contributor.alternativeName송봉석-
dc.contributor.alternativeName김선욱-
dc.contributor.alternativeName김민규-
dc.contributor.alternativeName심보웅-
dc.identifier.bibliographicCitationAntioxidants, vol. 10, no. 2, pp. 230-230-
dc.identifier.doi10.3390/antiox10020230-
dc.subject.keywordLycopene-
dc.subject.keywordAntioxidant-
dc.subject.keywordReactive oxygen species-
dc.subject.keywordMitochondria-dependent apoptosis-
dc.subject.keywordPig-
dc.subject.keywordReproduction-
dc.subject.localLycopene-
dc.subject.localAntioxidant-
dc.subject.localReactive oxygen species-
dc.subject.localReactive oxygen species (ROS)-
dc.subject.localPig-
dc.subject.localReproduction-
dc.description.journalClassY-
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > National Primate Research Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
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