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- Development of a novel nannochloropsis strain with enhanced violaxanthin yield for large-scale production
- Su Bin Park; Jin-Ho Yun; Ae Jin Ryu; Joohyun Yun; Ji Won Kim; Sujin Lee; Saehae Choi; Dae Hyun Cho; Dong Yun Choi; Yong Jae Lee; Hee-Sik Kim
- Bibliographic Citation
- Microbial Cell Factories, vol. 20, pp. 43-43
- Publication Year
- Background: Nannochloropsis is a marine microalga that has been extensively studied. The major carotenoid produced by this group of microalgae is violaxanthin, which exhibits anti-inflammatory, anti-photoaging, and antiproliferative activities. Therefore, it has a wide range of potential applications. However, large-scale production of this pigment has not been much studied, thereby limiting its industrial application.
Results: To develop a novel strain producing high amount of violaxanthin, various Nannochloropsis species were isolated from seawater samples and their violaxanthin production potential were compared. Of the strains tested, N. oceanica WS-1 exhibited the highest violaxanthin productivity; to further enhance the violaxanthin yield of WS-1, we performed gamma-ray-mediated random mutagenesis followed by colorimetric screening. As a result, Mutant M1 was selected because of its significant higher violaxanthin content and biomass productivity than WS-1 (5.21 ± 0.33 mg g- 1 and 0.2101 g L- 1 d- 1, respectively). Subsequently, we employed a 10 L-scale bioreactor to confirm the large-scale production potential of M1, and the results indicated a 43.54 % increase in violaxanthin production compared with WS-1. In addition, comparative transcriptomic analysis performed under normal light condition identified possible mechanisms associated with remediating photo-inhibitory damage and other key responses in M1, which seemed to at least partially explain enhanced violaxanthin content and delayed growth.
Conclusions: Nannochloropsis oceanica mutant (M1) with enhanced violaxanthin content was developed and its physiological characteristics were investigated. In addition, enhanced production of violaxanthin was demonstrated in the large-scale cultivation. Key transcriptomic responses that are seemingly associated with different physiological responses of M1 were elucidated under normal light condition, the details of which would guide ongoing efforts to further maximize the industrial potential of violaxanthin producing strains.
- ViolaxanthinMicroalgaeNannochloropsis oceanicaRandom mutagenesisTranscriptomeGamma-ray irradiation
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- Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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