Telomeres reforged with non-telomeric sequences in mouse embryonic stem cells

Cited 4 time in scopus
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Title
Telomeres reforged with non-telomeric sequences in mouse embryonic stem cells
Author(s)
Chuna Kim; S Sung; J S Kim; H Lee; Y Jung; S Shin; E Kim; J J Seo; J Kim; D Kim; H Niida; V N Kim; D Park; J Lee
Bibliographic Citation
Nature Communications, vol. 12, pp. 1097-1097
Publication Year
2021
Abstract
Telomeres are part of a highly refined system for maintaining the stability of linear chromosomes. Most telomeres rely on simple repetitive sequences and telomerase enzymes to protect chromosomal ends; however, in some species or telomerase-defective situations, an alternative lengthening of telomeres (ALT) mechanism is used. ALT mainly utilises recombination-based replication mechanisms and the constituents of ALT-based telomeres vary depending on models. Here we show that mouse telomeres can exploit non-telomeric, unique sequences in addition to telomeric repeats. We establish that a specific subtelomeric element, the mouse template for ALT (mTALT), is used for repairing telomeric DNA damage as well as for composing portions of telomeres in ALT-dependent mouse embryonic stem cells. Epigenomic and proteomic analyses before and after ALT activation reveal a high level of non-coding mTALT transcripts despite the heterochromatic nature of mTALT-based telomeres. After ALT activation, the increased HMGN1, a non-histone chromosomal protein, contributes to the maintenance of telomere stability by regulating telomeric transcription. These findings provide a molecular basis to study the evolution of new structures in telomeres.
ISSN
2041-1723
Publisher
Springer-Nature Pub Group
DOI
http://dx.doi.org/10.1038/s41467-021-21341-x
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
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