DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chang Kyu Heo | - |
dc.contributor.author | Hai Min Hwang | - |
dc.contributor.author | Won-Hee Lim | - |
dc.contributor.author | H J Lee | - |
dc.contributor.author | J S Yoo | - |
dc.contributor.author | K J Lim | - |
dc.contributor.author | Eun Wie Cho | - |
dc.date.accessioned | 2021-02-26T08:33:36Z | - |
dc.date.available | 2021-02-26T08:33:36Z | - |
dc.date.issued | 2020 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/24141 | - |
dc.description.abstract | Tumor-associated (TA) autoantibodies have been identified at the early tumor stage before developing clinical symptoms, which holds hope for early cancer diagnosis. We identified a TA autoantibody from HBx-transgenic (HBx-tg) hepatocellular carcinoma (HCC) model mouse, characterized its target antigen, and examined its relationship to human HCC. The mimotopes corresponding to the antigenic epitope of TA autoantibody were screened from a random cyclic peptide library and used for the detection of serum TA autoantibody. The target antigen of the TA autoantibody was identified as an oncogenic bi-functional purine biosynthesis protein, ATIC. It was upregulated in liver cancer tissues of HBx-tg mouse as well as human HCC tissues. Over-expressed ATIC was also secreted extracellularly via the cancer-derived exosomes, which might cause auto-immune responses. The cyclic peptide mimotope with a high affinity to anti-ATIC autoantibody, CLPSWFHRC, distinguishes between serum samples from HCC patients and healthy subjects with 70.83% sensitivity, 90.68% specificity (AUC = 0.87). However, the recombinant human ATIC protein showed a low affinity to anti-ATIC autoantibody, which may be incompatible as a capture antigen for serum TA autoantibody. This study indicates that anti-ATIC autoantibody can be a potential HCC-associated serum biomarker and suggests that autoantibody biomarker's efficiency can be improved by using antigenic mimicry to native antigens present in vivo. | - |
dc.publisher | MDPI | - |
dc.title | Cyclic peptide mimotopes for the detection of serum anti-ATIC autoantibody biomarker in hepato-cellular carcinoma | - |
dc.title.alternative | Cyclic peptide mimotopes for the detection of serum anti-ATIC autoantibody biomarker in hepato-cellular carcinoma | - |
dc.type | Article | - |
dc.citation.title | International Journal of Molecular Sciences | - |
dc.citation.number | 24 | - |
dc.citation.endPage | 9718 | - |
dc.citation.startPage | 9718 | - |
dc.citation.volume | 21 | - |
dc.contributor.affiliatedAuthor | Chang Kyu Heo | - |
dc.contributor.affiliatedAuthor | Hai Min Hwang | - |
dc.contributor.affiliatedAuthor | Won-Hee Lim | - |
dc.contributor.affiliatedAuthor | Eun Wie Cho | - |
dc.contributor.alternativeName | 허창규 | - |
dc.contributor.alternativeName | 황해민 | - |
dc.contributor.alternativeName | 임원희 | - |
dc.contributor.alternativeName | 이혜정 | - |
dc.contributor.alternativeName | 유종신 | - |
dc.contributor.alternativeName | 임국진 | - |
dc.contributor.alternativeName | 조은위 | - |
dc.identifier.bibliographicCitation | International Journal of Molecular Sciences, vol. 21, no. 24, pp. 9718-9718 | - |
dc.identifier.doi | 10.3390/ijms21249718 | - |
dc.subject.keyword | Autoantibody biomarker | - |
dc.subject.keyword | ATIC | - |
dc.subject.keyword | Hepatocellular carcinoma | - |
dc.subject.keyword | Cyclic peptide mimotope | - |
dc.subject.keyword | Human serum ELISA | - |
dc.subject.local | Autoantibody biomarker | - |
dc.subject.local | ATIC | - |
dc.subject.local | Hepatocellular carcinomas | - |
dc.subject.local | Hepatocellular carcinoma (HCC) | - |
dc.subject.local | Hepatocellular carcinoma | - |
dc.subject.local | hepatocellular carcinoma (HCC) | - |
dc.subject.local | hepatocellular carcinoma | - |
dc.subject.local | Cyclic peptide mimotope | - |
dc.subject.local | Human serum ELISA | - |
dc.description.journalClass | Y | - |
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