Cyclic peptide mimotopes for the detection of serum anti-ATIC autoantibody biomarker in hepato-cellular carcinoma

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dc.contributor.authorChang Kyu Heo-
dc.contributor.authorHai Min Hwang-
dc.contributor.authorWon-Hee Lim-
dc.contributor.authorH J Lee-
dc.contributor.authorJ S Yoo-
dc.contributor.authorK J Lim-
dc.contributor.authorEun Wie Cho-
dc.date.accessioned2021-02-26T08:33:36Z-
dc.date.available2021-02-26T08:33:36Z-
dc.date.issued2020-
dc.identifier.issn1661-6596-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/24141-
dc.description.abstractTumor-associated (TA) autoantibodies have been identified at the early tumor stage before developing clinical symptoms, which holds hope for early cancer diagnosis. We identified a TA autoantibody from HBx-transgenic (HBx-tg) hepatocellular carcinoma (HCC) model mouse, characterized its target antigen, and examined its relationship to human HCC. The mimotopes corresponding to the antigenic epitope of TA autoantibody were screened from a random cyclic peptide library and used for the detection of serum TA autoantibody. The target antigen of the TA autoantibody was identified as an oncogenic bi-functional purine biosynthesis protein, ATIC. It was upregulated in liver cancer tissues of HBx-tg mouse as well as human HCC tissues. Over-expressed ATIC was also secreted extracellularly via the cancer-derived exosomes, which might cause auto-immune responses. The cyclic peptide mimotope with a high affinity to anti-ATIC autoantibody, CLPSWFHRC, distinguishes between serum samples from HCC patients and healthy subjects with 70.83% sensitivity, 90.68% specificity (AUC = 0.87). However, the recombinant human ATIC protein showed a low affinity to anti-ATIC autoantibody, which may be incompatible as a capture antigen for serum TA autoantibody. This study indicates that anti-ATIC autoantibody can be a potential HCC-associated serum biomarker and suggests that autoantibody biomarker's efficiency can be improved by using antigenic mimicry to native antigens present in vivo.-
dc.publisherMDPI-
dc.titleCyclic peptide mimotopes for the detection of serum anti-ATIC autoantibody biomarker in hepato-cellular carcinoma-
dc.title.alternativeCyclic peptide mimotopes for the detection of serum anti-ATIC autoantibody biomarker in hepato-cellular carcinoma-
dc.typeArticle-
dc.citation.titleInternational Journal of Molecular Sciences-
dc.citation.number24-
dc.citation.endPage9718-
dc.citation.startPage9718-
dc.citation.volume21-
dc.contributor.affiliatedAuthorChang Kyu Heo-
dc.contributor.affiliatedAuthorHai Min Hwang-
dc.contributor.affiliatedAuthorWon-Hee Lim-
dc.contributor.affiliatedAuthorEun Wie Cho-
dc.contributor.alternativeName허창규-
dc.contributor.alternativeName황해민-
dc.contributor.alternativeName임원희-
dc.contributor.alternativeName이혜정-
dc.contributor.alternativeName유종신-
dc.contributor.alternativeName임국진-
dc.contributor.alternativeName조은위-
dc.identifier.bibliographicCitationInternational Journal of Molecular Sciences, vol. 21, no. 24, pp. 9718-9718-
dc.identifier.doi10.3390/ijms21249718-
dc.subject.keywordAutoantibody biomarker-
dc.subject.keywordATIC-
dc.subject.keywordHepatocellular carcinoma-
dc.subject.keywordCyclic peptide mimotope-
dc.subject.keywordHuman serum ELISA-
dc.subject.localAutoantibody biomarker-
dc.subject.localATIC-
dc.subject.localHepatocellular carcinomas-
dc.subject.localHepatocellular carcinoma (HCC)-
dc.subject.localHepatocellular carcinoma-
dc.subject.localhepatocellular carcinoma (HCC)-
dc.subject.localhepatocellular carcinoma-
dc.subject.localCyclic peptide mimotope-
dc.subject.localHuman serum ELISA-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
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