Downregulation of swpa4 peroxidase expression in transgenic sweetpotato plants decreases abiotic stress tolerance and reduces stress-related peroxidase expression

Cited 4 time in scopus
Metadata Downloads
Title
Downregulation of swpa4 peroxidase expression in transgenic sweetpotato plants decreases abiotic stress tolerance and reduces stress-related peroxidase expression
Author(s)
Y H Kim; Ho Soo Kim; Sung-Chul Park; Chang Yoon Ji; J W Yang; H U Lee; Sang Soo Kwak
Bibliographic Citation
Plant Biotechnology Reports, vol. 15, pp. 69-76
Publication Year
2021
Abstract
Class III peroxidases are involved in plant responses to various environmental conditions. Peroxidase production is triggered by various stimuli and activates stress-related signaling pathways to help plants cope with abiotic and biotic stresses. Recent evidence suggests that the interplay between diverse peroxidases plays a critical role in regulating stress responses. However, the underlying molecular mechanisms remain poorly understood. Previous research showed that transgenic sweetpotato overexpressing the swpa4 peroxidase gene exhibited increased tolerance to oxidative stress and high salinity. In this study, loss of function of the swpa4 peroxidase gene in sweetpotato was assessed in transgenic plants with constitutive RNAi-mediated suppression of the swpa4 gene. Compared with control plants, leaf discs from RNAi-suppressed lines exhibited increased damage following treatment with the oxidative stressors hydrogen peroxide and methyl viologen. Transgenic plants also showed reduced resistance to high salinity conditions compared with control plants. Repression of swpa4 also led to reduced expression of the endogenous stress-related peroxidase genes swpa1, swpa2, and swpn1. These findings suggest that swpa4 regulates signaling pathways involved in the regulation of peroxidase metabolism during the stress response in sweetpotato.
Keyword
High salinityOxidative stressPeroxidaseReactive oxygen speciesSweetpotato
ISSN
1863-5466
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11816-021-00660-x
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.