Alnus hirsuta (Spach) Rupr. attenuates airway inflammation and mucus overproduction in a murine model of ovalbumin-challenged asthma

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Title
Alnus hirsuta (Spach) Rupr. attenuates airway inflammation and mucus overproduction in a murine model of ovalbumin-challenged asthma
Author(s)
Ba Wool Lee; Ji Hye Ha; Yeongseon Ji; Seong Hun Jeong; Ju Hong Kim; Jihye Lee; Ji Young ParkHyung-Jun KwonKyungsook Jung; J C Kim; Young Bae RyuIn Chul Lee
Bibliographic Citation
Frontiers in Pharmacology, vol. 12, pp. 614442-614442
Publication Year
2021
Abstract
Alnus hirsuta (Spach) Rupr. (AH), a member of the Betulaceae family, is widely used in Eastern Asia of as a source of medicinal compounds for the treatment of hemorrhage, diarrhea, and alcoholism. In this study, we investigated the protective effects of a methanolic extract of AH branches against airway inflammation and mucus production in tumor necrosis factor (TNF)-α-stimulated NCI-H292 cells and in an ovalbumin (OVA)-challenged allergic asthma mouse model. Female BALB/c mice were injected with OVA (40 μg) and aluminum hydroxide (2 mg) on days 0 and 14 to induce allergic airway inflammation. The mice were then challenged with 1% OVA from days 21?23. Mice were treated with AH (50 and 100 mg/kg/day; 2% DMSO) or dexamethasone (positive control; 3 mg/kg/day) from days 18?23. AH treatment effectively attenuated airway resistance/hyperresponsiveness and reduced levels of T helper type 2 (Th2) cytokines, eotaxins, and number of inflammatory cells in bronchoalveolar lavage fluid, and immunoglobulin E in serums of OVA-challenged mice. In histological analysis, AH treatment significantly inhibited airway inflammation and mucus production in OVA-challenged mice. AH treatment downregulated the phosphorylation of I kappa B-alpha, p65 nuclear factor-kappa B (p65NF-κB), and mitogen-activated protein kinases with suppression of mucin 5AC (MUC5AC) in lung tissue. Moreover, AH treatment decreased the levels of pro-inflammatory cytokines and Th2 cytokines, as well as MUC5AC expression, and inhibited the phosphorylation of p65NF-κB in TNF-α-stimulated NCI-H292 cells. These results indicate that AH might represent a useful therapeutic agent for the treatment of allergic asthma.
Keyword
Allergic asthmaAirway inflammationMucus overproductionMitogen-activated protein (MAP) kinaseNuclear factor-kappa BAlnus hirsuta (spach) Rupr.
ISSN
1663-9812
Publisher
Frontiers Media Sa
DOI
http://dx.doi.org/10.3389/fphar.2021.614442
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
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