Calf diarrhea caused by prolonged expansion of autochthonous gut Enterobacteriaceae and their lytic bacteriophages

Cited 11 time in scopus
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Title
Calf diarrhea caused by prolonged expansion of autochthonous gut Enterobacteriaceae and their lytic bacteriophages
Author(s)
T W Whon; H S Kim; Na-Ri Shin; H Sung; M S Kim; J Y Kim; W Kang; P S Kim; D W Hyun; H J Seong; W J Sul; S W Rho; J W Bae
Bibliographic Citation
Msystems, vol. 6, no. 2, pp. e00816-20-e00816-20
Publication Year
2021
Abstract
Neonatal calf diarrhea is a common disease leading to a major economic loss for cattle producers worldwide. Several infectious and noninfectious factors are implicated in calf diarrhea, but disease control remains problematic because of the multifactorial etiology of the disease. Here, we conducted diagnostic multiplex PCR assay and meta-omics analysis (16S rRNA gene-based metataxonomics and untargeted transcriptional profiling) of rectal content of normal and diarrheic beef calves (n=111). In the diarrheic calf gut, we detected both microbial compositional dysbiosis (i.e., increased abundances of the family Enterobacteriaceae members and their lytic bacteriophages) and functional dysbiosis (i.e., elevated levels of aerobic respiration and virulence potential). The calf diarrheic transcriptome mirrored the gene expression of the bovine host and was enriched in cellular pathways of sulfur metabolism, innate immunity, and gut motility. We then isolated 12 nontoxigenic Enterobacteriaceae strains from the gut of diarrheic calves. Feeding a strain mixture to preweaning mice resulted in a significantly higher level of fecal moisture content, with decreased body weight gain and shortened colon length. The presented findings suggest that gut inflammation followed by a prolonged expansion of nontoxigenic autochthonous Enterobacteriaceae contributes to the onset of diarrhea in preweaning animals.
Keyword
Calf diarrheaGut microbiomeDysbiosisEnterobacteriaceaeBacteriophages
ISSN
2379-5077
Publisher
Amer Soc Microb
DOI
http://dx.doi.org/10.1128/mSystems.00816-20
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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