13R,20-dihydroxydocosahexaenoic acid, a novel dihydroxy-DHA derivative, inhibits breast cancer stemness through regulation of the Stat3/IL-6 signaling pathway by inducing ROS production = PUFA 대사체 13R,20-dH DHA의 ROS-STAT3 신호전달계를 통한 cancer stemness 제어

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dc.contributor.authorLifang Wang-
dc.contributor.authorH S Choi-
dc.contributor.authorBinna Lee-
dc.contributor.authorJong Hyun Choi-
dc.contributor.authorY S Jang-
dc.contributor.authorJeong-Woo Seo-
dc.date.accessioned2021-03-19T03:30:20Z-
dc.date.available2021-03-19T03:30:20Z-
dc.date.issued2021-
dc.identifier.issn2076-3921-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/24188-
dc.description.abstractBreast cancer is a major health problem worldwide. Cancer stem cells (CSCs) are known to mediate breast cancer metastasis and recurrence and are therefore a promising therapeutic target. In this study, we investigated the anti-inflammatory effect of 13R,20-dihydroxydocosahexaenoic acid (13R,20-diHDHA), a novel dihydroxy-DHA derivative, which was synthesized through an enzymatic reaction using cyanobacterial lipoxygenase. We found that 13R,20-diHDHA reduced the macrophage secretion of the inflammatory cytokines, IL-6 and TNF-α, and thus appeared to have anti-inflammatory effects. As the inflammatory tumor microenvironment is largely devoted to supporting the cancer stemness of breast cancer cells, we investigated the effect of 13R,20-diHDHA on breast cancer stemness. Indeed, 13R,20-diHDHA effectively inhibited breast cancer stemness, as evidenced by its ability to dose-dependently inhibit the mammospheres formation, colony formation, migration, and invasion of breast CSCs. 13R,20-diHDHA reduced the populations of CD44high/CD24low and aldehyde dehydrogenase (ALDH)-positive cells and the expression levels of the cancer stemness-related self-renewal genes, Nanog, Sox2, Oct4, c-Myc, and CD44. 13R,20-diHDHA increased reactive oxygen species (ROS) production, and the generated ROS reduced the phosphorylation of nuclear signal transducer and activator of transcription 3 (Stat3) and the secretion of IL-6 by mammospheres. These data collectively suggest that 13R,20-diHDHA inhibits breast cancer stemness through ROS production and downstream regulation of Stat3/IL-6 signaling, and thus might be developed as an anti-cancer agent acting against CSCs.-
dc.publisherMDPI-
dc.title13R,20-dihydroxydocosahexaenoic acid, a novel dihydroxy-DHA derivative, inhibits breast cancer stemness through regulation of the Stat3/IL-6 signaling pathway by inducing ROS production = PUFA 대사체 13R,20-dH DHA의 ROS-STAT3 신호전달계를 통한 cancer stemness 제어-
dc.title.alternative13R,20-dihydroxydocosahexaenoic acid, a novel dihydroxy-DHA derivative, inhibits breast cancer stemness through regulation of the Stat3/IL-6 signaling pathway by inducing ROS production-
dc.typeArticle-
dc.citation.titleAntioxidants-
dc.citation.number3-
dc.citation.endPage457-
dc.citation.startPage457-
dc.citation.volume10-
dc.contributor.affiliatedAuthorLifang Wang-
dc.contributor.affiliatedAuthorBinna Lee-
dc.contributor.affiliatedAuthorJong Hyun Choi-
dc.contributor.affiliatedAuthorJeong-Woo Seo-
dc.contributor.alternativeName왕리팡-
dc.contributor.alternativeName최학선-
dc.contributor.alternativeName이빛나-
dc.contributor.alternativeName최종현-
dc.contributor.alternativeName장용석-
dc.contributor.alternativeName서정우-
dc.identifier.bibliographicCitationAntioxidants, vol. 10, no. 3, pp. 457-457-
dc.identifier.doi10.3390/antiox10030457-
dc.subject.keywordBreast cancer stem cells-
dc.subject.keyword13R,20-dihydroxydocosahexaenoic acid (13R,20-diHDHA)-
dc.subject.keywordMammospheres-
dc.subject.keywordROS-
dc.subject.keywordStat3-
dc.subject.localBreast cancer stem cells-
dc.subject.local13R,20-dihydroxydocosahexaenoic acid (13R,20-diHDHA)-
dc.subject.localMammospheres-
dc.subject.localReactive oxidative species-
dc.subject.localReactive oxygen species(ROS)-
dc.subject.localReactive oxygen species-
dc.subject.localReactive Oxygen Species (ROS)-
dc.subject.localReactive Oxygen Species-
dc.subject.localROS-
dc.subject.localReactive oxygen species (ROS)-
dc.subject.localreactive oxygen species-
dc.subject.localreactive oxygen species (ROS)-
dc.subject.localSignal transducer and activator of transcription 3 (STAT3)-
dc.subject.localSignal transducer and activator of transcription-
dc.subject.localSignal transducer and activator of transcription 3 (Stat3)-
dc.subject.localSTAT 3-
dc.subject.localSTAT3-
dc.subject.localSignal transducer and activator of transcription 3-
dc.subject.localStat3-
dc.subject.localSignal transducer and activator of transcription factor 3 (STAT3)-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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