Neobacillus endophyticus sp. nov., an endophytic bacterium isolated from Selaginella involvens roots

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Title
Neobacillus endophyticus sp. nov., an endophytic bacterium isolated from Selaginella involvens roots
Author(s)
L Jiang; Myoung Hui Lee; Jae Cheol Jeong; D H Kim; Cha Young KimSuk Weon KimJiyoung Lee
Bibliographic Citation
International Journal of Systematic and Evolutionary Microbiology, vol. 71, no. 3, pp. 004581-004581
Publication Year
2021
Abstract
A Gram-stain-positive, facultatively anaerobic, rod-shaped, endospore-forming, oxidase-positive, and catalase-negative strain designated as BRMEA1T was isolated from the surface-sterilized Selaginella involvens roots. Growth of strain BRMEA1T was found to occur at pH 6.0-8.0 (optimum, pH 7.0), 15-50°C (optimum, 25-30°C) and in the absence of NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BRMEA1T formed a lineage within the genus Neobacillus (family Bacillaceae ) and showed the highest sequence similarity to Neobacillus drentensis DSM 15600T (98.3%) and Neobacillus fumarioli KCTC 13885T (98.2%), and less than 98.2% 16S rRNA gene sequence similarity to the other members of the genus Neobacillus . Whole-genome analysis of strain BRMEA1T comprised a circular chromosome (5 632 809 bp in size) with 38.5 mol% G+C content. Digital DNA-DNA hybridization analyses revealed that strain BRMEA1T showed 20.5 and 22.0% genomic DNA relatedness with the closest species, N. drentensis DSM 15600T and N. fumarioli KCTC 13885T, respectively. The whole-genome sequence of strain BRMEA1T showed the presence of 11 specific conserved signature indels for the genus Neobacillus . The major cellular fatty acids (>10%) of strain BRMEA1T were found to be iso-C15:0 and anteiso-C15:0, while the major polar lipids were found to be diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Polyphasic analysis results revealed that BRMEA1T represents a novel species of the genus Neobacillus , with the proposed name Neobacillus endophyticus sp. nov. The type strain is BRMEA1T (=KCTC 43208T=CCTCC AB 2020071T).
Keyword
Polyphasic taxonomyNovel speciesEndophytic bacteriumPhylogenyNeobacillus endophyticus
ISSN
1466-5026
Publisher
Microbiology Soc
DOI
http://dx.doi.org/10.1099/ijsem.0.004581
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Jeonbuk Branch Institute > 1. Journal Articles
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