Global proteomics to study silica nanoparticle-induced cytotoxicity and is mechanisms in HepG2 cells

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dc.contributor.authorS Y Lee-
dc.contributor.authorI Y Kim-
dc.contributor.authorM B Heo-
dc.contributor.authorJeong Hee Moon-
dc.contributor.authorJ G Son-
dc.contributor.authorT G Lee-
dc.date.accessioned2021-04-06T03:30:51Z-
dc.date.available2021-04-06T03:30:51Z-
dc.date.issued2021-
dc.identifier.issn2218-273X-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/24240-
dc.description.abstractSilica nanoparticles (SiO2 NPs) are commonly used in medical and pharmaceutical fields. Research into the cytotoxicity and overall proteomic changes occurring during initial exposure to SiO2 NPs is limited. We investigated the mechanism of toxicity in human liver cells according to exposure time [0, 4, 10, and 16 h (h)] to SiO2 NPs through proteomic analysis using mass spectrometry. SiO2 NP-induced cytotoxicity through various pathways in HepG2 cells. Interestingly, when cells were exposed to SiO2 NPs for 4 h, the morphology of the cells remained intact, while the expression of proteins involved in mRNA splicing, cell cycle, and mitochondrial function was significantly downregulated. These results show that the toxicity of the nanoparticles affects protein expression even if there is no change in cell morphology at the beginning of exposure to SiO2 NPs. The levels of reactive oxygen species changed significantly after 10 h of exposure to SiO2 NPs, and the expression of proteins associated with oxidative phosphorylation, as well as the immune system, was upregulated. Eventually, these changes in protein expression induced HepG2 cell death. This study provides insights into cytotoxicity evaluation at early stages of exposure to SiO2 NPs through in vitro experiments.-
dc.publisherMDPI-
dc.titleGlobal proteomics to study silica nanoparticle-induced cytotoxicity and is mechanisms in HepG2 cells-
dc.title.alternativeGlobal proteomics to study silica nanoparticle-induced cytotoxicity and is mechanisms in HepG2 cells-
dc.typeArticle-
dc.citation.titleBiomolecules-
dc.citation.number3-
dc.citation.endPage375-
dc.citation.startPage375-
dc.citation.volume11-
dc.contributor.affiliatedAuthorJeong Hee Moon-
dc.contributor.alternativeName이선영-
dc.contributor.alternativeName김인영-
dc.contributor.alternativeName허민범-
dc.contributor.alternativeName문정희-
dc.contributor.alternativeName손진경-
dc.contributor.alternativeName이태걸-
dc.identifier.bibliographicCitationBiomolecules, vol. 11, no. 3, pp. 375-375-
dc.identifier.doi10.3390/biom11030375-
dc.subject.keywordSilica nanoparticles-
dc.subject.keywordCytotoxicity-
dc.subject.keywordLabel-free quantification-
dc.subject.keywordProteomic analysis-
dc.subject.keywordMass spectrometry-
dc.subject.localSilica nanoparticle-
dc.subject.localsilica nanoparticles-
dc.subject.localSilica nanoparticles-
dc.subject.localsilica nanoparticle-
dc.subject.localCytotoxicity-
dc.subject.localcytotoxicity-
dc.subject.localLabel-free quantification-
dc.subject.localProteomic analyses-
dc.subject.localProteomic analysis-
dc.subject.localproteomic analysis-
dc.subject.localMass spetrometry-
dc.subject.localMass spectrometry (MS)-
dc.subject.localmass spectrometry-
dc.subject.localMass spectrometry-
dc.subject.localmass spectrometry (MS)-
dc.description.journalClassY-
Appears in Collections:
Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
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