V-set and immunoglobulin domain-containing 1 (Vsig1), predominantly expressed in testicular germ cells, is dispensable for spermatogenesis and male fertility in mice

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Title
V-set and immunoglobulin domain-containing 1 (Vsig1), predominantly expressed in testicular germ cells, is dispensable for spermatogenesis and male fertility in mice
Author(s)
Yena Jeong; H Bang; Young Hyun Kim; N E Park; Young-Ho Park; C Park; S R Lee; Jeong Woong LeeBong-Seok SongJi-Su KimBo Woong Sim; D W Seol; G Wee; S Kim; Sun-Uk Kim; E Kim
Bibliographic Citation
Animals, vol. 11, no. 4, pp. 1037-1037
Publication Year
2021
Abstract
To elucidate the functional role of V-set and immunoglobulin domain-containing 1 (VSIG1) in spermatogenesis and fertilization, we knocked out (KO) VSIG1 in a mouse embryo using CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9) -mediated genome editing. Reverse transcription PCR was performed using cDNA synthesized from VSIG1 KO testis RNA. Although Western blot analysis using a specific antibody to VSIG1 confirmed VSIG1 protein defects in the KO mice, hematoxylin-eosin staining analysis was similar in the KO and wild-type mice. Additionally, computer-assisted sperm analysis and in vitro fertilization experiments were conducted to confirm the activity and fertilization ability of sperm derived from the KO mouse. Mice lacking VSIG1 were viable and had no serious developmental defects. As they got older, the KO mice showed slightly higher weight loss, male mice lacking VSIG1 had functional testes, including normal sperm number and motility, and both male and female mice lacking VSIG1 were fertile. Our results from VSIG1 KO mice suggest that VSIG1 may not play essential roles in spermatogenesis and normal testis development, function, and maintenance. VSIG1 in sperm is dispensable for spermatogenesis and male fertility in mice. As several genes are known to possess slightly different functions depending on the species, the importance and molecular mechanism of VSIG1 in tissues of other species needs further investigation.
Keyword
SpermatogenesisFertilityVSIG1FertilizationSperm
ISSN
2076-2615
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/ani11041037
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > National Primate Research Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
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